[霍乱噬菌体DNA的分子量及限制性图谱分析]
[Molecular weight and restriction mapping of the DNA of cholera phages].
作者信息
Degtiarev B M, Marchenkov V I, Skopich A A, Arutiunov Iu I
出版信息
Mol Gen Mikrobiol Virusol. 1988 Feb(2):17-20.
PMID:3374504
Abstract
Molecular masses of cholera bacteriophages 493, 7226 and Eltor II were defined by electron microscopic technique. DNA of these bacteriophages was digested by the restriction endonucleases PstI, BglI, MluI and SalI. The number and molecular masses of the obtained restricts were identified. The physical map of bacteriophage 493 was constructed using three restriction endonucleases. The obtained data can be used for classification and molecular biology research of cholera bacteriophages.
摘要
通过电子显微镜技术测定了霍乱噬菌体493、7226和Eltor II的分子质量。这些噬菌体的DNA用限制性内切酶PstI、BglI、MluI和SalI进行消化。鉴定了所得限制性片段的数量和分子质量。使用三种限制性内切酶构建了噬菌体493的物理图谱。所得数据可用于霍乱噬菌体的分类和分子生物学研究。
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