HFRR, Throckmorton Kansas State University Manhattan, Manhattan, KS, 66506, USA.
Department of Plant Pathology, Throckmorton Kansas State University Manhattan, Manhattan, KS, 66506, USA.
Sci Rep. 2017 Sep 28;7(1):12377. doi: 10.1038/s41598-017-12355-x.
Tomatoes are known to have ameliorative effects on cardiovascular disease and cancer. The nutritional value of tomatoes can be enhanced by increasing flavonoids content through genetic modification. The regulatory gene PAP1 (production of anthocyanin pigment 1) from Arabidopsis is reported to increase initial flavonoid flux and anthocyanin content. The structural gene CHI from Alium cepa increases flavonol content. However, the number of structural genes that can be transferred to plants is limited. To solve this problem, for the first time, we produced gene stacking transgenic tomato, in which Arabidopsis PAP1 (production of anthocyanin pigment 1) was stacked with an onion CHI by crossing. This procedure resulted in increased rutin and total anthocyanin content of as much as 130 and 30 times more, respectively, than the content in wild tomato skin, compared with 2.3 and 3 times more flavonol content, and 1 and 1.5 times more anthocyanin content in unstacked FLS and PAP1 tomatoes, respectively.
西红柿被认为对心血管疾病和癌症具有改善作用。通过遗传修饰增加类黄酮含量可以提高西红柿的营养价值。拟南芥的调控基因 PAP1（花青素色素 1 的产生）据报道可以增加初始类黄酮通量和花青素含量。洋葱的结构基因 CHI 增加类黄酮醇含量。然而，可转移到植物中的结构基因数量是有限的。为了解决这个问题，我们首次生产了基因叠加转基因番茄，其中通过杂交将拟南芥 PAP1（花青素色素 1 的产生）与洋葱 CHI 叠加。与未叠加的 FLS 和 PAP1 番茄相比，这一过程使野生番茄皮中的芦丁和总花青素含量分别增加了 130 倍和 30 倍，而类黄酮醇含量分别增加了 2.3 倍和 3 倍，花青素含量分别增加了 1 倍和 1.5 倍。
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