Mayerhofer Johanna, Eckard Sonja, Hartmann Martin, Grabenweger Giselher, Widmer Franco, Leuchtmann Adrian, Enkerli Jürg
Molecular Ecology, Agroscope, CH-8046 Zurich, Switzerland.
Ecology of Noxious and Beneficial Organisms, Agroscope, CH-8046 Zurich, Switzerland.
FEMS Microbiol Ecol. 2017 Oct 1;93(10). doi: 10.1093/femsec/fix117.
The release of large quantities of microorganisms to soil for purposes such as pest control or plant growth promotion may affect the indigenous soil microbial communities. In our study, we investigated potential effects of Metarhizium brunneum ART2825 on soil fungi and prokaryota in bulk soil using high-throughput sequencing of ribosomal markers. Different formulations of this strain, and combinations of the fungus with garlic as efficacy-enhancing agent, were tested over 4 months in a pot and a field experiment carried out for biological control of Agriotes spp. in potatoes. A biocontrol effect was observed only in the pot experiment, i.e. the application of FCBK resulted in 77% efficacy. Colony counts combined with genotyping and marker sequence abundance confirmed the successful establishment of the applied strain. Only the formulated applied strain caused small shifts in fungal communities in the pot experiment. Treatment effects were in the same range as the effects caused by barley kernels, the carrier of the FCBK formulation and temporal effects. Garlic treatments and time affected prokaryotic communities. In the field experiment, only spatial differences affected fungal and prokaryotic communities. Our findings suggest that M. brunneum may not adversely affect soil microbial communities.
为了害虫防治或促进植物生长等目的而向土壤中释放大量微生物可能会影响土壤中的原生微生物群落。在我们的研究中，我们使用核糖体标记的高通量测序技术，研究了球孢白僵菌ART2825对大田土壤中真菌和原核生物的潜在影响。在为期4个月的盆栽和田间试验中，测试了该菌株的不同配方，以及该真菌与大蒜作为增效剂的组合，以对马铃薯中的金针虫进行生物防治。仅在盆栽试验中观察到了生物防治效果，即施用真菌载体制剂（FCBK）的防治效果达77%。菌落计数结合基因分型和标记序列丰度证实了所施用菌株的成功定殖。在盆栽试验中，只有配方化的施用菌株导致真菌群落发生了微小变化。处理效果与FCBK制剂的载体大麦粒所造成的影响以及时间效应处于同一范围。大蒜处理和时间影响原核生物群落。在田间试验中，只有空间差异影响真菌和原核生物群落。我们的研究结果表明，球孢白僵菌可能不会对土壤微生物群落产生不利影响。
