Salem Ali, Abdelhedi Ola, Ben Taheur Fadia, Mansour Chalbia, Safta Skhiri Sihem, Sebai Hichem, Jridi Mourad, Zouari Nacim, Fakhfakh Nahed
Laboratory of Functional Physiology and Valorization of Bio-resources (LR17ES27), Higher Institute of Biotechnology of Beja (ISBB), University of Jendouba, 9000, Beja, Tunisia.
High Institute of Applied Biology of Medenine, University of Gabes, 4119, Medenine, Tunisia.
Heliyon. 2024 Jun 17;10(12):e33048. doi: 10.1016/j.heliyon.2024.e33048. eCollection 2024 Jun 30.
This study aimed to investigate the ability of aqueous extract of seeds (LSE) to improve the wound healing process in rat models. The gelatin, extracted from the skin of smooth-hound shark using citric acid, was used as a support material for ointment. Animals were divided into four groups of six rats each: an untreated control group, a control group treated with Moist Exposed Burn Ointment (MEBO), a treated group with gelatin gel, and a treated group with gelatin gel fortified with 20 mg/mL LSE. Phenolics profile analysis showed that the major compounds in LSE were catechin (125 μg/g) and quinic acid (105 μg/g). antioxidant tests showed that LSE has interesting activities to scavenge ABTS•+ radicals (IC = 0.22 mg/mL) and inhibit the oxidation of linoleic acid. A significant decline in the antioxidant enzymes activities and an increase in the level of thiobarbituric acid reactive substances (TBARS) and inflammatory markers was observed within the injured tissues of the untreated rats compared to rats treated with MEBO. Interestingly, when the wounded tissue was treated with gelatin gel a remarkable reversal of this trend occurred. Further, by enrichment of gelatin gel with LSE, the levels of CAT, GPx and SOD activities significantly increased by 35, 126, and 212 %, respectively, whereas the TBARS level was reduced by 31 %. These results were consistent with the wound contraction percentage and histological analysis, which suggest the potential effect of LSE-enriched gelatin gels to regenerate damaged tissues.
本研究旨在探究种子水提取物（LSE）改善大鼠模型伤口愈合过程的能力。使用柠檬酸从光鳞鲨皮肤中提取的明胶作为软膏的支撑材料。将动物分为四组，每组六只大鼠：未处理的对照组、用湿润烧伤膏（MEBO）处理的对照组、用明胶凝胶处理的组以及用含20 mg/mL LSE强化的明胶凝胶处理的组。酚类物质谱分析表明，LSE中的主要化合物是儿茶素（125 μg/g）和奎尼酸（105 μg/g）。抗氧化试验表明，LSE具有清除ABTS•+自由基（IC = 0.22 mg/mL）和抑制亚油酸氧化的有趣活性。与用MEBO处理的大鼠相比，在未处理大鼠的受伤组织中观察到抗氧化酶活性显著下降，硫代巴比妥酸反应性物质（TBARS）水平和炎症标志物增加。有趣的是，当用明胶凝胶处理受伤组织时，这种趋势发生了显著逆转。此外，通过用LSE富集明胶凝胶，CAT、GPx和SOD活性水平分别显著增加了35%、126%和212%，而TBARS水平降低了31%。这些结果与伤口收缩百分比和组织学分析一致，表明富含LSE的明胶凝胶对再生受损组织具有潜在作用。
