School of Chemistry and Environment, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou, China.
School of Chemistry and Environment, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou, China.
Biosens Bioelectron. 2018 Feb 15;100:482-489. doi: 10.1016/j.bios.2017.09.028. Epub 2017 Sep 20.
The rapid detection of antibiotic residual in everyday life is very important for food safety. In order to realize the on-site and visual detection of antibiotic, a POCT method was established by using digital image colorimetry based on smartphone. Streptomycin was taken as the analyte model of antibiotics, streptomycin aptamer preferentially recognized analyte, and the excess aptamer hybridized with the complementary DNA to form the dsDNA. SYBR Green I combined with the dsDNA and then emitted obvious green fluorescence, thus the fluorescence intensity decreased with the increasing of streptomycin concentration. Then a smartphone-based device was constructed as the fluorescence readout. The smartphone camera acquired the images of the fluorescence derived from the samples, and the Touch Color APP installed in smartphone read out the RGB values of the images. There was a linear relationship between the G values and the streptomycin concentrations in the range of 0.1-100µM. The detection limit was 94nM, which was lower than the maximum residue limit defined by World Health Organization. The POCT method was applied for determining streptomycin in chicken and milk samples with recoveries in 94.1-110%. This method had the advantages of good selectivity, simple operation and on-site visualization.
日常生活中快速检测抗生素残留非常重要，这关系到食品安全。为了实现抗生素的现场可视化检测，建立了一种基于智能手机的数字图像比色法 POCT 方法。以链霉素为抗生素分析物模型，链霉素适体优先识别分析物，多余的适体与互补 DNA 杂交形成 dsDNA。SYBR Green I 与 dsDNA 结合后发出明显的绿色荧光，因此荧光强度随链霉素浓度的增加而降低。然后构建了基于智能手机的设备作为荧光读出器。智能手机摄像头获取来自样品的荧光图像，智能手机上安装的 Touch Color APP 读取图像的 RGB 值。G 值与 0.1-100µM 范围内链霉素浓度之间存在线性关系。检测限为 94nM，低于世界卫生组织规定的最大残留限量。该 POCT 方法用于测定鸡肉和牛奶样品中的链霉素，回收率在 94.1-110%之间。该方法具有选择性好、操作简单、现场可视化等优点。
