免疫相关基因IL17F和IL10的遗传变异与功能性消化不良相关：一项病例对照研究。
Genetic variants of immune-related genes IL17F and IL10 are associated with functional dyspepsia: A case-control study.
作者信息
Singh Rajan, Ghoshal Uday C, Kumar Sushil, Mittal Balraj
机构信息
Department of Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, India.
Department of Medical Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, India.
出版信息
Indian J Gastroenterol. 2017 Sep;36(5):343-352. doi: 10.1007/s12664-017-0788-7. Epub 2017 Sep 30.
BACKGROUND
Low-grade inflammation may play an important role in pathogenesis of functional dyspepsia (FD). Since cytokines may influence gastric mucosal inflammation, which is associated with FD, we evaluated singe nucleotide polymorphisms (SNPs) of pro-inflammatory IL17F and anti-inflammatory IL10 cytokine genes in patients with FD and healthy subjects (HS).
METHODS
Two hundred and thirty-seven consecutive patients with FD (Rome III) and 250 HS were genotyped for IL17F (rs2397084: A/G, rs763780: T/C) and IL10 (rs1800896: G/A, rs1800871: C/T) (PCR-RFLP).
RESULTS
Patients with FD [173 (73%) men, age 38.4±12 years] were comparable with HS [195 (78%) men, age 37.3±12 years] with respect to age and gender. Out of 237 patients, 26 (11%) had epigastric pain, 55 (23.2%) had post-prandial distress syndromes (EPS, PDS), and 156 (65.8%) had EPS-PDS overlap. Among 237 patients with FD, GG (variant) genotype of IL17F (rs2397084) was more common than HS [15 (6.3%) vs. 4 (1.6%), p=0.015, odds ratio (OR)=4.0, 95% confidence interval (CI)=1.3-12.3]. IL17F (rs763780) and IL10 (rs1800896) were comparable among patients and HS (p=0.56, 0.28), respectively. However, TT (variant) genotype of IL10 (rs1800871) was more common among patients than HS [39 (16.5%) vs. 32 (12.8%), p=0.06, OR=1.7, 95% CI=0.98-2.98]. SNPs of IL17F and IL10 (rs2397084, rs763780, rs1800896 and rs1800871) were comparable among patients among sub-types of FD (p=0.80 and 0.44).
CONCLUSION
SNPs of IL17F (rs2397084) and IL10 (rs1800871) genes are associated with FD.
背景
低度炎症可能在功能性消化不良（FD）的发病机制中起重要作用。由于细胞因子可能影响与FD相关的胃黏膜炎症，我们评估了FD患者和健康受试者（HS）中促炎性白细胞介素17F（IL17F）和抗炎性白细胞介素10（IL10）细胞因子基因的单核苷酸多态性（SNP）。
方法
对237例连续的FD（罗马III型）患者和250例HS进行IL17F（rs2397084：A/G，rs763780：T/C）和IL10（rs1800896：G/A，rs1800871：C/T）基因分型（聚合酶链反应-限制性片段长度多态性法）。
结果
FD患者[173例（73%）为男性，年龄38.4±12岁]与HS[195例（78%）为男性，年龄37.3±12岁]在年龄和性别方面具有可比性。在237例患者中，26例（11%）有上腹部疼痛，55例（23.2%）有餐后不适综合征（EPS，PDS），156例（65.8%）有EPS-PDS重叠。在237例FD患者中，IL17F（rs2397084）的GG（变异）基因型比HS更常见[15例（6.3%）对4例（1.6%），p=0.015，比值比（OR）=4.0，95%置信区间（CI）=1.3-12.3]。IL17F（rs763780）和IL10（rs1800896）在患者和HS中分别具有可比性（p=0.56，0.28）。然而，IL10（rs1800871）的TT（变异）基因型在患者中比HS更常见[39例（16.5%）对32例（12.8%），p=0.06，OR=1.7，95%CI=0.98-2.98]。IL17F和IL10（rs2397084、rs763780、rs1800896和rs1800871）的SNP在FD各亚型患者中具有可比性（p=0.80和0.44）。
结论
IL17F（rs2397084）和IL10（rs1800871）基因的SNP与FD相关。
Zotero 插件