da Silva B R, Conrado A J S, Pereira A L, Evaristo F F V, Arruda F V S, Vasconcelos M A, Lorenzón E N, Cilli E M, Teixeira E H
a DPML/LIBS, Integrated Laboratory of Biomolecules , Federal University of Ceará , Fortaleza , Brazil.
d School of Dentistry , Universidade de Fortaleza - UNIFOR , Fortaleza , Brazil.
Biofouling. 2017 Nov;33(10):835-846. doi: 10.1080/08927014.2017.1374378. Epub 2017 Oct 2.
The aims of this study were to describe the synthesis of a novel synthetic peptide based on the primary structure of the KR-12 peptide and to evaluate its antimicrobial and anti-biofilm activities against Streptococcus mutans. The antimicrobial effect of KR-12 and [W]KR12-KAEK was assessed by determining the minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations. The evaluation of anti-biofilm activity was assessed through total biomass quantification, colony forming unit counting and scanning electron microscopy. [W]KR12-KAEK showed MIC and MBC values ranging from 31.25 to 7.8 and 62.5 to 15.6 μg ml, respectively. Furthermore, [W7]KR12-KAEK significantly reduced biofilm biomass (50-100%). Regarding cell viability, [W]KR12-KAEK showed reductions in the number of CFUs at concentrations ranging from 62.5 to 7.8 μg ml and 500 to 62.5 μg ml with respect to biofilm formation and preformed biofilms, respectively. SEM micrographs of S. mutans treated with [W]KR12-KAEK suggested damage to the bacterial surface. [W]KR12-KAEK is demonstrated to be an antimicrobial agent to control microbial biofilms.
本研究的目的是描述基于KR-12肽一级结构的新型合成肽的合成，并评估其对变形链球菌的抗菌和抗生物膜活性。通过测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）来评估KR-12和[W]KR12-KAEK的抗菌效果。通过总生物量定量、菌落形成单位计数和扫描电子显微镜评估抗生物膜活性。[W]KR12-KAEK的MIC和MBC值分别为31.25至7.8 μg/ml和62.5至15.6 μg/ml。此外，[W7]KR12-KAEK显著降低了生物膜生物量（50-100%）。关于细胞活力，[W]KR12-KAEK在浓度分别为62.5至7.8 μg/ml和500至62.5 μg/ml时，相对于生物膜形成和预先形成的生物膜，CFU数量减少。用[W]KR12-KAEK处理的变形链球菌的扫描电子显微镜照片显示细菌表面受损。[W]KR已经被证明是一种控制微生物生物膜的抗菌剂。
