Ma Yan, Sun Xuewu, Huang Kangmao, Shen Shuying, Lin Xianfeng, Xie Ziang, Wang Jiying, Fan Shunwu, Ma Jianjun, Zhao Xing
Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou 310016, China.
Key Laboratory of Biotherapy of Zhejiang Province, Hangzhou 310016, China.
Oncotarget. 2017 Apr 11;8(38):62900-62913. doi: 10.18632/oncotarget.17036. eCollection 2017 Sep 8.
Inflammatory cytokines play critical roles in the pathogenesis of osteoarthritis. Recent studies have demonstrated that natural active substances can serve as alternative therapeutic agents for the prevention and treatment of osteoarthritis. Sanguinarine, an alkaloid isolated from the roots of , is known to have anti-inflammatory properties. The aim of the present study was to investigate the therapeutic effect of Sanguinarine against osteoarthritis. Sanguinarine inhibited interleukin-1β-induced expression of matrix metalloproteinase 1, 3, and 13, and A disintegrin and metalloproteinase with thrombospondin motifs-5 in chondrocytes, which involved the nuclear factor-κB and c-Jun N-terminal kinase signalling pathways. Furthermore, the study of interleukin-1β-induced cartilage matrix degradation in an anterior cruciate ligament transection-induced osteoarthritis model revealed that Sanguinarine ameliorated osteoarthritis by inhibiting the expression of matrix metalloproteinase 1, 3, and 13, and A disintegrin and metalloproteinase with thrombospondin motifs-5. In conclusion, we demonstrated for the first time that Sanguinarine suppressed the expression of matrix metalloproteinase 1, 3, and 13, and A disintegrin and metalloproteinase with thrombospondin motifs-5 , , and , indicating its potential usefulness in treating osteoarthritis.
炎症细胞因子在骨关节炎的发病机制中起关键作用。最近的研究表明，天然活性物质可作为预防和治疗骨关节炎的替代治疗剂。血根碱是一种从[植物名称]根中分离出的生物碱，已知具有抗炎特性。本研究的目的是探讨血根碱对骨关节炎的治疗作用。血根碱抑制白细胞介素-1β诱导的软骨细胞中基质金属蛋白酶1、3和13以及含血小板反应蛋白基序的解聚素和金属蛋白酶-5的表达，这涉及核因子-κB和c-Jun氨基末端激酶信号通路。此外，在交叉韧带切断诱导的骨关节炎模型中对白介素-1β诱导的软骨基质降解的研究表明，血根碱通过抑制基质金属蛋白酶1、3和13以及含血小板反应蛋白基序的解聚素和金属蛋白酶-5的表达来改善骨关节炎。总之，我们首次证明血根碱抑制基质金属蛋白酶1、3和13以及含血小板反应蛋白基序的解聚素和金属蛋白酶-5[具体物种]、[具体物种]和[具体物种]的表达，表明其在治疗骨关节炎方面的潜在用途。
