1 Department of Biology, Federico II University, Via Cintia, 80126 Naples, Italy.
2 Department of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, 86090 Pesche, Italy.
Benef Microbes. 2017 Oct 13;8(5):823-831. doi: 10.3920/BM2017.0025. Epub 2017 Oct 3.
A well-established rat model of diet-induced metabolic syndrome was used to evaluate the effects of the oral administration of spores or cells of HU16, a carotenoid-producing strain of Bacillus indicus. Symptoms of metabolic syndrome were induced in 90-days old, male Sprague-Dawley rats maintained for eight weeks on a high-fat diet, as previously reported. Parallel groups of animals under the same diet regimen also received a daily dose of 1×10 cells or spores of B. indicus HU16. Cells of strain HU16 were able to reduce symptoms of metabolic syndrome, plasma markers of inflammation and oxidative markers in plasma and liver to levels similar to those observed in rats under a standard diet. HU16 cells did not affect obesity markers or the accumulation of triglycerides in the liver of treated animals. Denaturing gradient gel electrophoresis analysis showed that the oral administration of HU16 cells did not significantly affect the gut microbiota of high fat-fed rats, suggesting that the observed beneficial effects are not due to a reshaping of the gut microbiota but rather to metabolites produced by HU16 cells.
采用一种成熟的饮食诱导代谢综合征大鼠模型，评估经口给予产类胡萝卜素的印度芽孢杆菌 HU16 孢子或细胞的效果。按照先前的报道，将 90 天大的雄性 Sprague-Dawley 大鼠维持 8 周高脂肪饮食，诱导代谢综合征症状。在相同饮食方案下的平行动物组还接受了每日 1×10 个细胞或孢子的印度芽孢杆菌 HU16 的剂量。菌株 HU16 细胞能够降低代谢综合征的症状、血浆炎症标志物和氧化标志物以及肝脏中的水平，达到与标准饮食下大鼠相似的水平。HU16 细胞对肥胖标志物或治疗动物肝脏中甘油三酯的积累没有影响。变性梯度凝胶电泳分析表明，HU16 细胞的口服给药并没有显著影响高脂肪喂养大鼠的肠道微生物群，这表明观察到的有益效果不是由于肠道微生物群的重塑，而是由于 HU16 细胞产生的代谢产物。
