Auberval Nathalie, Dal Stéphanie, Maillard Elisa, Bietiger William, Peronet Claude, Pinget Michel, Schini-Kerth Valérie, Sigrist Séverine
UMR DIATHEC, EA 7294, Centre Européen d'Etude du Diabète, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, Bld René Leriche, 67200, Strasbourg, France.
UMR 7175 CNRS, Pharmacologie et Physico-Chimie, Faculté de Pharmacie, Université de Strasbourg, 67400, Illkirch, France.
Eur J Nutr. 2017 Jun;56(4):1467-1475. doi: 10.1007/s00394-016-1192-2. Epub 2016 Feb 25.
Individuals with metabolic syndrome (MS) show several metabolic abnormalities including insulin resistance, dyslipidaemia, and oxidative stress (OS). Diet is one of the factors influencing the development of MS, and current nutritional advice emphasises the benefits of fruit and vegetable consumption. Here, we assessed the effects of naturally occurring antioxidants, red wine polyphenols (RWPs), on MS and OS.
Wistar rats (n = 20) weighing 200-220 g received a high-fat diet (HFD) for 2 months before they were divided into two groups that received either HFD only or HFD plus 50 mg/kg RWPs in their drinking water for an additional 2 months. A control group (n = 10) received a normal diet (ND) for 4 months.
Rats receiving HFD increased body weight over 20 % throughout the duration of the study. They also showed increased blood levels of C-peptide, glucose, lipid peroxides, and oxidised proteins. In addition, the HFD increased OS in hepatic, pancreatic, and vascular tissues, as well as induced pancreatic islet cell hyperplasia and hepatic steatosis. Addition of RWPs to the HFD attenuated these effects on plasma and tissue OS and on islet cell hyperplasia. However, RWPs had no effect on blood glucose levels or hepatic steatosis.
RWPs showed an antioxidant mechanism of action against MS. This result will inform future animal studies exploring the metabolic effects of RWPs in more detail. In addition, these findings support the use of antioxidants as adjunctive nutritional treatments for patients with diabetes.
患有代谢综合征（MS）的个体表现出多种代谢异常，包括胰岛素抵抗、血脂异常和氧化应激（OS）。饮食是影响MS发生发展的因素之一，目前的营养建议强调食用水果和蔬菜的益处。在此，我们评估了天然抗氧化剂红酒多酚（RWPs）对MS和OS的影响。
体重200 - 220克的Wistar大鼠（n = 20）先接受2个月的高脂饮食（HFD），然后分为两组，一组继续仅接受HFD，另一组在饮用水中添加50毫克/千克RWPs，再持续2个月。对照组（n = 10）接受4个月的正常饮食（ND）。
在整个研究期间，接受HFD的大鼠体重增加超过20%。它们还表现出血液中C肽、葡萄糖、脂质过氧化物和氧化蛋白水平升高。此外，HFD增加了肝脏、胰腺和血管组织中的OS，还诱导了胰岛细胞增生和肝脏脂肪变性。在HFD中添加RWPs减弱了这些对血浆和组织OS以及胰岛细胞增生的影响。然而，RWPs对血糖水平或肝脏脂肪变性没有影响。
RWPs显示出针对MS的抗氧化作用机制。这一结果将为未来更详细探索RWPs代谢作用的动物研究提供参考。此外，这些发现支持将抗氧化剂用作糖尿病患者的辅助营养治疗手段。
