Department of Respiratory Medicine, Shenzhen Children's Hospital, Shenzhen 518026, Guangdong Province, China.
WeHealthGene Institute, Shenzhen 518129, Guangdong Province, China.
World J Gastroenterol. 2017 Sep 7;23(33):6164-6171. doi: 10.3748/wjg.v23.i33.6164.
To investigate whether patients with refractory epilepsy and healthy infants differ in gut microbiota (GM), and how ketogenic diet (KD) alters GM.
A total of 14 epileptic and 30 healthy infants were recruited and seizure frequencies were recorded. Stool samples were collected for 16S rDNA sequencing using the Illumina Miseq platform. The composition of GM in each sample was analyzed with MOTHUR, and inter-group comparison was conducted by R software.
After being on KD treatment for a week, 64% of epileptic infants showed an obvious improvement, with a 50% decrease in seizure frequency. GM structure in epileptic infants (P1 group) differed dramatically from that in healthy infants (Health group). Proteobacteria, which had accumulated significantly in the P1 group, decreased dramatically after KD treatment (P2 group). Cronobacter predominated in the P1 group and remained at a low level both in the Health and P2 groups. Bacteroides increased significantly in the P2 group, in which Prevotella and Bifidobacterium also grew in numbers and kept increasing.
GM pattern in healthy infants differed dramatically from that of the epileptic group. KD could significantly modify symptoms of epilepsy and reshape the GM of epileptic infants.
探讨难治性癫痫患儿与健康婴儿的肠道菌群（GM）是否存在差异，以及生酮饮食（KD）如何改变 GM。
共招募 14 例癫痫患儿和 30 例健康婴儿，记录癫痫发作频率。使用 Illumina Miseq 平台对粪便样本进行 16S rDNA 测序。使用 MOTHUR 分析每个样本的 GM 组成，并通过 R 软件进行组间比较。
在 KD 治疗一周后，64%的癫痫患儿明显改善，癫痫发作频率降低 50%。癫痫患儿（P1 组）的 GM 结构与健康婴儿（健康组）明显不同。在 P1 组中显著积累的变形菌在 KD 治疗后（P2 组）急剧下降。在 P1 组中， Cronobacter 占优势，在健康组和 P2 组中均保持低水平。P2 组中的拟杆菌显著增加，其中普雷沃氏菌和双歧杆菌的数量也增加，并持续增加。
健康婴儿的 GM 模式与癫痫组有很大差异。KD 可显著改善癫痫症状，并重塑癫痫患儿的 GM。
