西班牙加那利群岛兰萨罗特岛采集的米勒（L.）微共生体Mlalz-1的高质量基因组草图序列。
High-quality draft genome sequence of Mlalz-1, a microsymbiont of (L.) miller collected in Lanzarote, Canary Islands, Spain.
作者信息
Osman Wan Adnawani Meor, van Berkum Peter, León-Barrios Milagros, Velázquez Encarna, Elia Patrick, Tian Rui, Ardley Julie, Gollagher Margaret, Seshadri Rekha, Reddy T B K, Ivanova Natalia, Woyke Tanja, Pati Amrita, Markowitz Victor, Baeshen Mohamed N, Baeshen Naseebh Nabeeh, Kyrpides Nikos, Reeve Wayne
机构信息
School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA Australia.
U.S. Department of Agriculture, Soybean Genomics and Improvement Laboratory, Beltsville Agricultural Research Center, 10300 Baltimore Avenue, Bldg. 006, Beltsville, MD 20705 USA.
出版信息
Stand Genomic Sci. 2017 Sep 25;12:58. doi: 10.1186/s40793-017-0270-2. eCollection 2017.
10.1601/nm.1335 Mlalz-1 (INSDC = ATZD00000000) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen-fixing nodule of (L.) Miller from a soil sample collected near the town of Guatiza on the island of Lanzarote, the Canary Islands, Spain. This strain nodulates and forms an effective symbiosis with the highly specific host . This rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) sequencing project. Here the features of 10.1601/nm.1335 Mlalz-1 are described, together with high-quality permanent draft genome sequence information and annotation. The 6,664,116 bp high-quality draft genome is arranged in 99 scaffolds of 100 contigs, containing 6314 protein-coding genes and 74 RNA-only encoding genes. Strain Mlalz-1 is closely related to 10.1601/nm.1335 10.1601/strainfinder?urlappend=%3Fid%3DIAM+12611 , 10.1601/nm.1334 A 321 and 10.1601/nm.17831 10.1601/strainfinder?urlappend=%3Fid%3DORS+1407 , based on 16S rRNA gene sequences. gANI values of ≥98.1% support the classification of strain Mlalz-1 as 10.1601/nm.1335. Nodulation of requires a specific allele, and the gene of strain Mlalz-1 shares ≥98% sequence identity with of -nodulating 10.1601/nm.1328 strains, but ≤93% with of 10.1601/nm.1328 strains that nodulate other species. Strain Mlalz-1 is unique among sequenced 10.1601/nm.1335 strains in possessing genes encoding components of a T2SS and in having two versions of the adaptive acid tolerance response operon. In 10.1601/nm.1334 strain 10.1601/strainfinder?urlappend=%3Fid%3DWSM+419, is essential for enhancing survival in lethal acid conditions. The second copy of the operon of strain Mlalz-1 has highest sequence identity (> 96%) with that of 10.1601/nm.1334 strains, which suggests genetic recombination between strain Mlalz-1 and 10.1601/nm.1334 and the horizontal gene transfer of .
10.1601/nm.1335 Mlalz - 1（国际核苷酸序列数据库协作组织编号 = ATZD00000000）是一种需氧、能运动、革兰氏阴性、不形成芽孢的杆菌，它是从西班牙加那利群岛兰萨罗特岛瓜蒂扎镇附近采集的土壤样本中，从豆科植物（L.）米勒的一个有效的固氮根瘤中分离出来的。该菌株能与高度特异性宿主形成根瘤并建立有效的共生关系。作为美国能源部联合基因组研究所2010年细菌和古细菌基因组百科全书 - 根瘤菌（GEBA - RNB）测序项目的一部分，对该根瘤菌的基因组进行了测序。这里描述了10.1601/nm.1335 Mlalz - 1的特征，以及高质量的永久草图基因组序列信息和注释。这个6,664,116 bp的高质量草图基因组排列在99个支架上，由100个重叠群组成，包含6314个蛋白质编码基因和74个仅编码RNA的基因。基于16S rRNA基因序列，Mlalz - 1菌株与10.1601/nm.1335 10.1601/strainfinder?urlappend=%3Fid%3DIAM + 12611、10.1601/nm.1334 A 321和10.1601/nm.17831 10.1601/strainfinder?urlappend=%3Fid%3DORS + 1407密切相关。≥98.1%的平均核苷酸一致性（gANI）值支持将Mlalz - 1菌株归类为10.1601/nm.1335。与宿主形成根瘤需要特定的等位基因，Mlalz - 1菌株的基因与能与宿主形成根瘤的10.1601/nm.1328菌株的基因序列同一性≥98%，但与能与其他宿主物种形成根瘤的10.1601/nm.1328菌株的基因序列同一性≤93%。在已测序的10.1601/nm.1335菌株中，Mlalz - 1菌株独特之处在于拥有编码II型分泌系统（T2SS）组件的基因，并且有两个版本的适应性耐酸反应操纵子。在10.1601/nm.1334菌株10.1601/strainfinder?urlappend=%3Fid%3DWSM + 419中，该操纵子对于在致死酸性条件下提高存活率至关重要。Mlalz - 1菌株的第二个操纵子拷贝与10.
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