Department of Public Health and Caring Sciences, Family Medicine and Preventive Medicine, Uppsala University, 751 22, Uppsala, Sweden.
Department of Molecular Medicine and Surgery, Karolinska Institutet, Solna, Sweden.
Cell Tissue Res. 2017 Dec;370(3):451-460. doi: 10.1007/s00441-017-2684-y. Epub 2017 Oct 3.
The role of inflammation and the mechanism of tendon healing after rupture has historically been a matter of controversy. The purpose of the present study is to investigate the role of mast cells and their relation to the NMDA receptor-1 (a glutamate receptor) during healing after Achilles tendon rupture. Eight female Sprague Dawley rats had their right Achilles tendon transected. Three weeks after rupture, histological quantification of mast cell numbers and their state of degranulation was assessed by histochemistry. Co-localization of mast cell tryptase (a mast cell marker) and NMDA receptor-1 was determined by immunofluorescence. The intact left Achilles tendon was used as control. An increased number of mast cells and a higher proportion of degranulated mast cells were found in the healing Achilles tendon compared to the intact. In addition, increased co-localization of mast cell tryptase and NMDA receptor-1 was seen in the areas of myotendinous junction, mid-tendon proper and bone tendon junction of the healing versus the intact tendon. These findings introduce a possible role for mast cells in the healing phase after Achilles tendon rupture.
炎症的作用以及肌腱断裂后愈合的机制一直存在争议。本研究旨在探讨肥大细胞及其与 NMDA 受体-1（一种谷氨酸受体）在跟腱断裂后的愈合过程中的关系。8 只雌性 Sprague Dawley 大鼠的右侧跟腱被切断。在断裂后 3 周，通过组织化学评估肥大细胞数量及其脱颗粒状态的组织学定量。通过免疫荧光测定肥大细胞胰蛋白酶（肥大细胞标志物）和 NMDA 受体-1 的共定位。将完整的左侧跟腱用作对照。与完整的跟腱相比，愈合的跟腱中发现了更多的肥大细胞和更高比例的脱颗粒肥大细胞。此外，在愈合的跟腱的肌腱连接、中腱本身和骨腱连接区域中，观察到肥大细胞胰蛋白酶和 NMDA 受体-1 的共定位增加。这些发现为肥大细胞在跟腱断裂后的愈合阶段可能发挥的作用提供了证据。
