Dias I J, Trajano E R I S, Castro R D, Ferreira G L S, Medeiros H C M, Gomes D Q C
Departamento de Odontologia, Universidade Estadual da Paraíba, Campina Grande, PB, Brazil.
Departamento de Odontologia, Universidade Federal da Paraíba, João Pessoa, PB, Brazil.
Braz J Biol. 2018 May;78(2):368-374. doi: 10.1590/1519-6984.171054. Epub 2017 Sep 28.
This study analyzed the antifungal activity of phytoconstituents from linalool on Candida spp. strains, in vitro, isolated from patients with clinical diagnoses of oral candidiasis associated with the use of a dental prosthesis. Biological samples were collected from 12 patients using complete dentures or removable partial dentures and who presented mucous with diffuse erythematous or stippled features, indicating a clinical diagnosis of candidiasis. To identify fungal colonies of the genus Candida, samples were plated onto CHROMagar Candida®. The antifungal activity of linalool, a monoterpene unsaturated constituent of basil oil, was performed using the broth microdilution technique. Then, the minimum inhibitory concentration (MIC), the two subsequent stronger concentrations and the positive controls were subcultured on Sabouraud Dextrose Agar plates to determine the minimum fungicidal concentration (MFC). The experiments were performed in triplicate and nystatin was used as a positive control in all tests. Diagnoses of oral candidiasis were verified in eight patients (66.6%) and the most prevalent fungal species was Candida albicans (37.5%), followed by Candida krusei (25.0%); and Candida tropicalis (4.2%). The best antifungal activity of linalool was observed on Candida tropicalis (MIC = 500 mg/mL), followed by Candida albicans (MIC = 1.000 mg/mL), and Candida krusei (MIC = 2.000 mg/mL).Under the study conditions and based on the results obtained, it can be concluded that the Candida strains tested were susceptible to linalool.
本研究分析了芳樟醇中植物成分对从临床诊断为与使用假牙相关的口腔念珠菌病患者中分离出的念珠菌属菌株的体外抗真菌活性。从12名使用全口假牙或可摘局部假牙且出现黏膜弥漫性红斑或点状特征（表明念珠菌病临床诊断）的患者中采集生物样本。为了鉴定念珠菌属的真菌菌落，将样本接种到CHROMagar Candida®培养基上。使用肉汤微量稀释技术检测了罗勒油中的单萜不饱和成分芳樟醇的抗真菌活性。然后，将最低抑菌浓度（MIC）、随后的两个更高浓度以及阳性对照在沙氏葡萄糖琼脂平板上进行传代培养，以确定最低杀菌浓度（MFC）。实验重复进行三次，所有试验均使用制霉菌素作为阳性对照。在8名患者（66.6%）中确诊为口腔念珠菌病，最常见的真菌种类是白色念珠菌（37.5%），其次是克柔念珠菌（25.0%）和热带念珠菌（4.2%）。观察到芳樟醇对热带念珠菌的抗真菌活性最佳（MIC = 500 mg/mL），其次是白色念珠菌（MIC = 1000 mg/mL）和克柔念珠菌（MIC = 2000 mg/mL）。在研究条件下并基于所得结果，可以得出结论，所测试的念珠菌菌株对芳樟醇敏感。
