Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia.
South Kalimantan Public Health Office, Banjarmasin, Indonesia.
J Trop Pediatr. 2018 Jun 1;64(3):208-214. doi: 10.1093/tropej/fmx051.
Blastocystis hominis is an enteric protozoan with many subtypes. It is frequently found in children and may cause chronic diarrhea. This study revealed Blastocystis subtypes among primary school children and comparison of molecular technique and culture method in Blastocystis diagnosis. A total of 141 stools were collected, examined microscopically, selected into the Blastocystis and negative parasite groups, for diagnostic comparison between culture and 18S rRNA polymerase chain reaction (PCR) methods. Positive PCR amplicons were subsequently sequenced for subtyping. The PCR results revealed 89%, 78%, 80% and 88% sensitivity, specificity and positive and negative predictive values, respectively, in comparison with the culture method (McNemar, p > 0.05). Sixteen PCR samples were successfully sequenced and resulted in three Blastocystis subtypes 1, 3 and 4. In conclusion, PCR was sensitive enough and can be used to exclude Blastocystis infection up to 88% of the cases. Subtypes 3 and 1 were the main subtypes found in apparently healthy school children in Jakarta.
人芽囊原虫是一种具有多种亚型的肠道原生动物。它在儿童中很常见，可能导致慢性腹泻。本研究揭示了小学生中的人芽囊原虫亚型，并比较了分子技术和培养方法在人芽囊原虫诊断中的应用。共采集了 141 份粪便，进行显微镜检查，将其分为人芽囊原虫和寄生虫阴性组，用于培养法和 18S rRNA 聚合酶链反应（PCR）方法的诊断比较。阳性 PCR 扩增子随后进行测序以进行亚型分型。与培养方法相比，PCR 结果分别显示出 89%、78%、80%和 88%的灵敏度、特异性和阳性及阴性预测值（McNemar，p>0.05）。16 个 PCR 样本成功测序，结果显示有三种人芽囊原虫亚型 1、3 和 4。总之，PCR 足够敏感，可用于排除 88%的人芽囊原虫感染病例。在雅加达的健康小学生中，主要发现了亚型 3 和 1。
