Liu Zunpeng, Zhu Yue, Ge Rui, Zhu Jiajun, He Xiaoning, Yuan Xue, Liu Xinchun
Department of Orthopedics, First Affiliated Hospital, China Medical University, Shenyang, China.
Department of Orthopedics, Fourth Affiliated Hospital, China Medical University, Shenyang, China.
Oncotarget. 2017 Jul 31;8(37):62298-62311. doi: 10.18632/oncotarget.19749. eCollection 2017 Sep 22.
Bone tissue engineering provides a substitute for bone transplantation in spinal fusion. This study examined if combined bone marrow-derived mesenchymal stem cells (BMSCs) sheet with platelet-rich plasma (PRP) could promote bone regeneration in a rabbit posterolateral spinal fusion model. BMSCs was isolated and confirmed by Flow cytometric analysis and immunofluorescence staining. The morphology of BMSCs was examined by Hematoxylin and Eosin staining, scanning and transmission electron microscopy. BMSCs were cultured in induction medium or control medium. The osteogenic ability of BMSCs was investigated by various histochemical staining, immunofluorescence staining and qRT-PCR analysis. The BMSCs/PRP was constructed by encapsulating the PRP block with BMSCs sheet. Twenty-four adult rabbits were randomly divided into four groups based on the implanted biomaterials: BMSCs/PRP, BMSCs, iliac crest autograft, and control group. Manual palpation and digital radiography analysis showed that the fusion rate was 100%, 0, 83.3%, and 0 in these 4 groups, respectively. Formation of continuous bone masses in BMSCs/PRP group was confirmed by computed tomography scanning and 3D-reconstruction. These studies demonstrated that BSMCs/PRP significantly accelerated bone regeneration in the rabbit posterolateral spinal fusion model.
骨组织工程为脊柱融合术中的骨移植提供了一种替代方法。本研究检测了骨髓间充质干细胞（BMSCs）片与富血小板血浆（PRP）联合应用是否能促进兔后外侧脊柱融合模型中的骨再生。通过流式细胞术分析和免疫荧光染色分离并鉴定BMSCs。通过苏木精和伊红染色、扫描及透射电子显微镜检查BMSCs的形态。将BMSCs培养于诱导培养基或对照培养基中。通过各种组织化学染色、免疫荧光染色和qRT-PCR分析研究BMSCs的成骨能力。通过用BMSCs片包裹PRP块构建BMSCs/PRP。根据植入的生物材料将24只成年兔随机分为四组：BMSCs/PRP组、BMSCs组、自体髂骨移植组和对照组。手法触诊和数字X线摄影分析显示，这4组的融合率分别为100%、0、83.3%和0。通过计算机断层扫描和三维重建证实BMSCs/PRP组形成了连续的骨块。这些研究表明，在兔后外侧脊柱融合模型中，BSMCs/PRP显著加速了骨再生。
