Childs Silas P, King Zachary R, Walker David R, Harris Donna K, Pedley Kerry F, Buck James W, Boerma H Roger, Li Zenglu
Department of Crop and Soil Sciences and Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, USA.
Soybean/Maize Germplasm, Pathology and Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Urbana, IL, USA.
Theor Appl Genet. 2018 Jan;131(1):27-41. doi: 10.1007/s00122-017-2983-4. Epub 2017 Oct 4.
A novel Rpp gene from PI 605823 for resistance to Phakopsora pachyrhizi was mapped on chromosome 19. Soybean rust, caused by the obligate biotrophic fungal pathogen Phakopsora pachyrhizi Syd. & P. Syd, is a disease threat to soybean production in regions of the world with mild winters. Host plant resistance conditioned by resistance to P. pachyrhizi (Rpp) genes has been found in numerous soybean accessions, and at least 10 Rpp genes or alleles have been mapped to six genetic loci. Identifying additional disease-resistance genes will facilitate development of soybean cultivars with durable resistance. PI 605823, a plant introduction from Vietnam, was previously identified as resistant to US populations of P. pachyrhizi in greenhouse and field trials. In this study, bulked segregant analysis using an F population derived from 'Williams 82' × PI 605823 identified a genomic region associated with resistance to P. pachyrhizi isolate GA12, which had been collected in the US State of Georgia in 2012. To further map the resistance locus, linkage mapping was carried out using single-nucleotide polymorphism markers and phenotypic data from greenhouse assays with an F population derived from Williams 82 × PI 605823 and an F population derived from '5601T' × PI 605823. A novel resistance gene, Rpp7, was mapped to a 154-kb interval (Gm19: 39,462,291-39,616,643 Glyma.Wm82.a2) on chromosome 19 that is different from the genomic locations of any previously reported Rpp genes. This new gene could be incorporated into elite breeding lines to help provide more durable resistance to soybean rust.
来自PI 605823的一个对大豆锈菌具有抗性的新Rpp基因被定位在19号染色体上。由专性活体营养型真菌病原菌大豆锈菌（Phakopsora pachyrhizi Syd. & P. Syd）引起的大豆锈病，对世界上冬季温和地区的大豆生产构成病害威胁。在众多大豆种质资源中发现了由对大豆锈菌（Rpp）基因的抗性所决定的寄主植物抗性，并且至少有10个Rpp基因或等位基因已被定位到6个遗传位点。鉴定更多的抗病基因将有助于培育具有持久抗性的大豆品种。PI 605823是一份从越南引进的植物材料，先前在温室和田间试验中被鉴定为对美国大豆锈菌群体具有抗性。在本研究中，利用从‘Williams 82’×PI 605823衍生的F群体进行集群分离分析，鉴定出一个与对2012年在美国佐治亚州采集的大豆锈菌分离株GA12的抗性相关的基因组区域。为了进一步定位抗性位点，利用单核苷酸多态性标记和来自温室试验的表型数据，对从Williams 82×PI 605823衍生的F群体以及从‘5601T’×PI 605823衍生的F群体进行连锁作图。一个新的抗性基因Rpp7被定位到19号染色体上一个154 kb的区间（Gm19: 39,462,291 - 39,616,643 Glyma.Wm82.a2），该区间不同于任何先前报道的Rpp基因的基因组位置。这个新基因可以被整合到优良育种系中，以帮助提供对大豆锈病更持久的抗性。
