双微体染色体可由染色体缺失产生的前体形成。
Double minute chromosomes can be produced from precursors derived from a chromosomal deletion.
作者信息
Carroll S M, DeRose M L, Gaudray P, Moore C M, Needham-Vandevanter D R, Von Hoff D D, Wahl G M
机构信息
Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037.
出版信息
Mol Cell Biol. 1988 Apr;8(4):1525-33. doi: 10.1128/mcb.8.4.1525-1533.1988.
Recent experiments have shown that gene amplification can be mediated by submicroscopic, autonomously replicating, circular extrachromosomal molecules. We refer to those molecules as episomes (S. Carroll, P. Gaudray, M. L. DeRose, J. F. Emery, J. L. Meinkoth, E. Nakkim, M. Subler, D. D. Von Hoff, and G. M. Wahl, Mol. Cell. Biol. 7:1740-1750, 1987). The experiments reported in this paper explore the way episomes are formed and their fate in the cell over time. The data reveal that in our system the episomes are initially 250 kilobases, but gradually enlarge until they become double minute chromosomes. In addition, we show that episomes or double minute chromosomes can integrate into chromosomes. Our results also suggest that episomes can be produced by deletion of the corresponding sequences from the chromosome.
最近的实验表明，基因扩增可由亚显微的、自主复制的环状染色体外分子介导。我们将这些分子称为附加体（S. 卡罗尔、P. 高德里、M. L. 德罗丝、J. F. 埃默里、J. L. 迈因科斯、E. 纳基姆、M. 苏布勒、D. D. 冯·霍夫和G. M. 瓦尔，《分子细胞生物学》7:1740 - 1750，1987年）。本文报道的实验探讨了附加体的形成方式及其在细胞中的长期命运。数据显示，在我们的系统中，附加体最初为250千碱基，但会逐渐增大，直至成为双微体染色体。此外，我们表明附加体或双微体染色体可整合到染色体中。我们的结果还表明，附加体可通过从染色体上删除相应序列而产生。
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