染色体位置对动物细胞中转染基因扩增的影响。
Effect of chromosomal position on amplification of transfected genes in animal cells.
作者信息
Wahl G M, Robert de Saint Vincent B, DeRose M L
出版信息
Nature. 1984;307(5951):516-20. doi: 10.1038/307516a0.
DOI:10.1038/307516a0
PMID:6694743
Abstract
A single protein (CAD) contains the first three enzymatic activities of de novo uridine biosynthesis. The chromosomal location of CAD genes introduced into Chinese hamster ovary cells significantly affects the frequency and cytogenetic result of their amplification. The amplification frequency in one transformant is 100-fold that of the others; in another, amplification of donated genes inserted near a centromere results in chromosome instability and rearrangements.
摘要
一种单一蛋白质（CAD）具备从头合成尿苷的前三种酶活性。导入中国仓鼠卵巢细胞的CAD基因的染色体定位会显著影响其扩增频率和细胞遗传学结果。一个转化体中的扩增频率是其他转化体的100倍；在另一个转化体中，插入着丝粒附近的供体基因的扩增导致染色体不稳定和重排。
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