Department of Chemistry, Soonchunhyang University, Asan 31538, Republic of Korea.
SH Company, Asan 31538, Republic of Korea.
Acta Biochim Biophys Sin (Shanghai). 2017 Oct 1;49(10):945-953. doi: 10.1093/abbs/gmx090.
Orostachys japonicus is an herb that contains several functional components and has traditionally been used to treat various diseases in Asia. In this study, bioactive components from different parts of the O. japonicus plant were investigated, and the contents of the functional components in ethanol extracts of O. japonicus cultivated in Korea and China were compared. The antioxidant effects of O. japonicus ethanol extracts were investigated using Raw 264.7 cells. It was found that 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity was significantly decreased in the cells treated with the extracts. Moreover, the novel inhibitory functions of O. japonicus extracts on collagenase, elastase, and tyrosinase were established. We also found that O. japonicus extracts strongly inhibited melanin synthesis in B16F10 melanoma cells by decreasing MITF protein levels and activating the Erk and Akt signaling pathways. Thus, these findings would be useful for developing new cosmetic and pharmaceutical formulations based on O. japonicus extracts.
瓦松是一种草本植物，含有多种功能成分，在亚洲传统上被用于治疗各种疾病。本研究对瓦松植物不同部位的生物活性成分进行了研究，并比较了韩国和中国栽培的瓦松乙醇提取物中功能成分的含量。利用 Raw 264.7 细胞研究了瓦松乙醇提取物的抗氧化作用。结果发现，提取物处理的细胞 2,2-二苯基-1-苦基肼自由基清除活性显著降低。此外，还确定了瓦松提取物对胶原酶、弹性蛋白酶和酪氨酸酶的新型抑制作用。我们还发现，瓦松提取物通过降低 MITF 蛋白水平和激活 Erk 和 Akt 信号通路，强烈抑制 B16F10 黑色素瘤细胞中的黑色素合成。因此，这些发现将有助于开发基于瓦松提取物的新型化妆品和药物制剂。
