通过布里渊显微镜测量的选择性双光子胶原交联
Selective two-photon collagen crosslinking measured by Brillouin microscopy.
作者信息
Kwok Sheldon J J, Kuznetsov Ivan A, Kim Moonseok, Choi Myunghwan, Scarcelli Giuliano, Yun Seok Hyun
机构信息
Harvard-MIT Division of Health Sciences and Technology, 77 Massachusetts Avenue, Cambridge, MA 02139.
Wellman Center for Photomedicine, Massachusetts General Hospital, 50 Blossom Street, Boston MA 02114.
出版信息
Optica. 2016;3(5):469-472. doi: 10.1364/OPTICA.3.000469. Epub 2016 May 4.
Two-photon polymerization has enabled precise microfabrication of three-dimensional structures with applications spanning from photonic microdevices, drug delivery systems, and cellular scaffolds. We present two-photon collagen crosslinking (2P-CXL) of intact corneal tissue using riboflavin and femtosecond laser irradiation. Collagen fiber orientations and photobleaching were characterized by second harmonic generation and two-photon fluorescence imaging, respectively. Measurement of local changes in longitudinal mechanical moduli with confocal Brillouin microscopy enabled the visualization of the cross-linked pattern without perturbation of the surrounding non-irradiated regions. 2P-CXL induced stiffening was comparable to that achieved with conventional one-photon CXL. Our results demonstrate the ability to selectively stiffen biological tissue at high resolution with broad implications in ophthalmology, laser surgery, and tissue engineering.
双光子聚合技术已实现了三维结构的精确微加工，其应用涵盖光子微器件、药物递送系统和细胞支架等领域。我们展示了使用核黄素和飞秒激光照射对完整角膜组织进行双光子胶原交联（2P-CXL）。分别通过二次谐波产生和双光子荧光成像对胶原纤维取向和光漂白进行了表征。利用共焦布里渊显微镜测量纵向力学模量的局部变化，能够在不干扰周围未照射区域的情况下可视化交联模式。2P-CXL诱导的硬化与传统单光子CXL所达到的效果相当。我们的结果证明了以高分辨率选择性地使生物组织硬化的能力，这在眼科、激光手术和组织工程中具有广泛的意义。
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