Kwon Yong Min, Kim Kyung Woo, Kim Jaoon Young Hwan, Choi Tae-Young, Yang Sung-Hyun, Oh Chul Hong, Kwon Kae Kyoung, Kim Sang-Jin
Department of Applied Research, National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101beon-gil, Seocheon-gun, Chungcheongnam-do, 33662, Republic of Korea.
Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101beon-gil, Seocheon-gun, Chungcheongnam-do, 33662, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Mar;68(3):764-768. doi: 10.1099/ijsem.0.002581. Epub 2018 Feb 6.
A Gram-negative, yellow-pigmented, non-flagellated, gliding, rod-shaped and aerobic bacterium, designated MEBiC 12267, was isolated from green algae of Jeju Island. 16S rRNA gene sequence analysis revealed that the strain MEBiC 12267 was affiliated to the genus Euzebyella of the family Flavobacteriaceae and showed the highest similarity to Euzebyella marina KCTC 42440 (98.5 %). The DNA-DNA relatedness value of strain MEBiC 12267 with E. marina KCTC 42440 was 25 %. Growth was observed at 10-37 °C (optimum, 30-33 °C), at pH 6.0-9.5 (optimum, 8.0-8.5) and with 0.5-9.0 % (w/v) NaCl (optimum, 2.5-3.5 %). The predominant cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH. The major respiratory quinone was MK-6. Polar lipids included phosphatidylethanolamine, seven unidentified lipids and two unidentified aminolipids. The DNA G+C content was 40.7 mol%. On the basis of the data from the polyphasic taxonomic study, it was concluded that the strain MEBiC 12267 represents a novel species within the genus Euzebyella, for which the name Euzebyella algicola sp. nov. is proposed. The type strain of E. algicola is MEBiC 12267 (=KCCM 43264=JCM 32170).
从济州岛的绿藻中分离出一株革兰氏阴性、产黄色色素、无鞭毛、滑行、杆状的需氧细菌，命名为MEBiC 12267。16S rRNA基因序列分析表明，菌株MEBiC 12267隶属于黄杆菌科的Euzebyella属，与滨海Euzebyella菌KCTC 42440的相似性最高（98.5%）。菌株MEBiC 12267与滨海Euzebyella菌KCTC 42440的DNA-DNA相关性值为25%。在10-37 °C（最适温度为30-33 °C）、pH 6.0-9.5（最适pH为8.0-8.5）和0.5-9.0%（w/v）NaCl（最适浓度为2.5-3.5%）条件下可观察到生长。主要的细胞脂肪酸为异-C15 : 0、异-C15 : 1 G和异-C17 : 0 3-OH。主要呼吸醌为MK-6。极性脂质包括磷脂酰乙醇胺、七种未鉴定的脂质和两种未鉴定的氨基脂质。DNA G+C含量为40.7 mol%。基于多相分类研究的数据，得出结论：菌株MEBiC 12267代表Euzebyella属内的一个新物种，为此提出新名称Euzebyella algicola sp. nov.。嗜藻Euzebyella菌的模式菌株为MEBiC 12267（=KCCM 43264=JCM 32170）。
