sp. nov., isolated from marine alga ( (L.) Gaillon).

A novel Gram-stain-negative, straight or curved rod-shaped, non-spore-forming, strictly aerobic, motile bacterium with a single polar flagellum, designated D3211, was isolated from marine alga collected at the seashore of Yantai, PR China. The organism grew optimally at 24 °C, pH 7.0 and in the presence of 2.0 % (w/v) NaCl. Strain D3211 contained ubiquinone 8 as the major respiratory quinone and C 7 and/or C 6, C, iso-C and anteiso-C B and/or iso-C I as the major fatty acids. The predominant polar lipids of strain D3211 were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strain D3211 was 39.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to BSs20135, JDTF-33, KCTC 32108, DSM 15026, JCM 13954 and ARK 150 with 97.6, 97.6, 97.5, 97.4, 97.3 and 97.1 % sequence similarities, respectively. Calculated average nucleotide identity and DNA-DNAhybridization values between strain D3211 and its phylogenetically related species were in the range 70.2-73.4 % and 19.1-20.4 %, respectively. On the basis of polyphasic analyses, strain D3211 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D3211 (=KCTC 72122=MCCC 1K03603).