Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China.
Bioresour Technol. 2018 Jan;247:610-615. doi: 10.1016/j.biortech.2017.09.133. Epub 2017 Sep 21.
The present study showed that inhibition of autophagy significantly increased cellular levels of reactive oxygen species in Chlorella zofingiensis under nitrogen starvation. This was accompanied with increased expression of PSY, and enhanced accumulation of astaxanthin after 48h of cultivation. Nevertheless, the proportion of astaxanthin in secondary carotenoids remained unchanged. Meanwhile, the expression level of ACCase was also elevated in the 3-MA-treated cells compared to the control despite a >20% lower content of fatty acid in the former than the latter. This phenomenon might be due to inhibition of recycling of cellular components by 3-MA and suggests the potential involvement of post-transcriptional regulation in fatty acid biosynthesis. In summary, our work has been the first to report a potentially important role of autophagy in fatty acid and astaxanthin accumulation in C. zofingiensis under stress conditions. The findings might provide valuable insights to guide further research in this area.
本研究表明，在氮饥饿条件下，自噬抑制显著增加了小球藻细胞内活性氧的水平。这伴随着 PSY 的表达增加，以及培养 48 小时后虾青素的积累增加。然而，类胡萝卜素的虾青素比例保持不变。同时，与对照组相比，在 3-MA 处理的细胞中 ACCase 的表达水平也升高，尽管前者的脂肪酸含量比后者低 20%以上。这种现象可能是由于 3-MA 抑制了细胞成分的循环利用，并表明脂肪酸生物合成中可能涉及转录后调控。总之，我们的工作首次报道了自噬在小球藻应激条件下脂肪酸和虾青素积累中的重要作用。这一发现可能为指导该领域的进一步研究提供有价值的见解。
