Sweeney G E, Old R W
Department of Biological Sciences, University of Warwick, Coventry, UK.
Nucleic Acids Res. 1988 Jun 10;16(11):4903-13. doi: 10.1093/nar/16.11.4903.
To study factors required for immunoglobulin gene transcription hybrid promoters were made by linking octamer elements to a Xenopus albumin gene construct containing only 50bp of the albumin gene promoter. When injected into oocytes these hybrid promoters directed transcription far less efficiently than the unmodified 50bp albumin gene promoter fragment. Activity of the hybrid promoter, but not the unmodified albumin promoter, could be stimulated by preinjection of poly(A)+ RNA from NS1 myeloma cells. This stimulation may be caused by translation of the NS1 poly(A)+ RNA into transcription factors that act on the octamer. Both the reduction in transcription caused by octamer insertion and the extent of the inducibility by NS1 RNA are greater when two, rather than one, octamers are inserted.
为了研究免疫球蛋白基因转录所需的因子，通过将八聚体元件连接到仅包含50bp白蛋白基因启动子的非洲爪蟾白蛋白基因构建体上，构建了杂交启动子。当将这些杂交启动子注射到卵母细胞中时，其指导转录的效率远低于未修饰的50bp白蛋白基因启动子片段。杂交启动子而非未修饰的白蛋白启动子的活性，可以通过预先注射NS1骨髓瘤细胞的聚腺苷酸加尾RNA（poly(A)+ RNA）来刺激。这种刺激可能是由于NS1 poly(A)+ RNA翻译为作用于八聚体的转录因子所致。当插入两个而非一个八聚体时，由八聚体插入导致的转录减少以及NS1 RNA的诱导程度都更大。
