Old R W, Sheikh S A, Chambers A, Newton C A, Mohammed A, Aldridge T C
Nucleic Acids Res. 1985 Oct 25;13(20):7341-58. doi: 10.1093/nar/13.20.7341.
We have assessed the response of many histone H3 mRNAs and an H1C mRNA in Xenopus tissue culture cells after treatment with the DNA synthesis inhibitor hydroxyurea. The amount of the histone mRNAs falls rapidly in response to the inhibitor. This response is prevented by cycloheximide. Cloned Xenopus histone genes were transfected into mouse cells and a cell line was obtained in which the Xenopus genes were actively expressed giving rise to mRNA with correct 5'-termini. The Xenopus genes were correctly regulated at the level of mRNA amounts in the mouse cell line. Nuclear microinjection experiments with Xenopus oocytes and S1 nuclease analysis of normal ovary RNA showed that the H1C gene, and probably also two H3 genes, which are replication-dependent in somatic cells are expressed in oocytes and are therefore replication-independent in this cell type. The same promoters are used in both replication-dependent and independent expression.
我们评估了非洲爪蟾组织培养细胞在用DNA合成抑制剂羟基脲处理后，多种组蛋白H3 mRNA和一种H1C mRNA的反应。组蛋白mRNA的量会因抑制剂而迅速下降。这种反应可被环己酰亚胺阻止。将克隆的非洲爪蟾组蛋白基因转染到小鼠细胞中，获得了一个细胞系，其中非洲爪蟾基因被积极表达，产生具有正确5'末端的mRNA。非洲爪蟾基因在小鼠细胞系中mRNA量的水平上得到了正确调控。用非洲爪蟾卵母细胞进行的核显微注射实验以及对正常卵巢RNA的S1核酸酶分析表明，在体细胞中依赖复制的H1C基因，可能还有两个H3基因，在卵母细胞中表达，因此在这种细胞类型中是不依赖复制的。在依赖复制和不依赖复制的表达中使用的是相同的启动子。
