Madrahimov Nodir, Natanov Ruslan, Boyle Erin C, Goecke Tobias, Knöfel Ann-Kathrin, Irkha Valentyna, Solovieva Anna, Höffler Klaus, Maus Ulrich, Kühn Christian, Ismail Issam, Warnecke Gregor, Shrestha Malakh-Lal, Cebotari Serghei, Haverich Axel
Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School;
Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School.
J Vis Exp. 2017 Sep 22(127):56017. doi: 10.3791/56017.
As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization and for minimizing organ damage resulting from prolonged extracorporal circulation. The goal of this paper was to demonstrate a fully functional and clinically relevant model of cardiopulmonary bypass in a mouse. We report on the device design, perfusion circuit optimization, and microsurgical techniques. This model is an acute model, which is not compatible with survival due to the need for multiple blood drawings. Because of the range of tools available for mice (e.g., markers, knockouts, etc.), this model will facilitate investigation into the molecular mechanisms of organ damage and the effect of cardiopulmonary bypass in relation to other comorbidities.
随着在心脏介入手术中长时间体外循环变得越发必要，临床对手术优化以及将长时间体外循环导致的器官损伤降至最低的需求也日益增加。本文的目的是展示一种在小鼠身上具备完整功能且与临床相关的体外循环模型。我们报告了该装置的设计、灌注回路优化以及显微外科技术。此模型是一个急性模型，由于需要多次采血，所以与存活不兼容。鉴于有多种适用于小鼠的工具（例如，标记物、基因敲除等），该模型将有助于研究器官损伤的分子机制以及体外循环与其他合并症相关的影响。
