Dequin S, Gloeckler R, Herbert C J, Boutelet F
TEPRAL S.A., Strasbourg, France.
Curr Genet. 1988 Jun;13(6):471-8. doi: 10.1007/BF02427752.
The ERG10 gene specific to S. uvarum, a brewing yeast, has been cloned by complementation of an S. cerevisiae erg10 mutant. S. uvarum contains two different ERG10 genes. One of these is similar to the S. cerevisiae ERG10 gene; they are structurally different, but functionally homologous. The cloned ERG10 gene has been located on chromosome XVI, and we have shown that it is allelic to the previously isolated tsm0115 mutants. Northern blot and sequence analysis indicate that the ERG10 gene is highly expressed, and biochemical and genetic evidence show that it encodes the cytoplasmic acetoacetyl CoA thiolase.
通过对酿酒酵母erg10突变体进行互补，克隆出了酿酒酵母特有的ERG10基因。酿酒酵母含有两个不同的ERG10基因。其中一个与酿酒酵母的ERG10基因相似；它们在结构上不同，但功能同源。克隆出的ERG10基因位于第十六条染色体上，并且我们已经证明它与之前分离出的tsm0115突变体是等位基因。Northern印迹和序列分析表明ERG10基因高度表达，生化和遗传学证据表明它编码细胞质乙酰乙酰辅酶A硫解酶。
