通过三体分析对酿酒酵母中的 arg、cpa、car 和 tsm 基因进行遗传定位。

Genetic mapping of arg, cpa, car and tsm genes in Saccharomyces cerevisiae by trisomic analysis.

机构信息

Chaire de Microbiologie, Faculté des Sciences Agronomiques de l'État, 5800, Gembloux, Belgium.

出版信息

Curr Genet. 1982 Nov;6(2):93-8. doi: 10.1007/BF00435206.

Abstract

By use of a set of 8 aneuploid strains of the yeast Saccharomyces cerevisiae, carrying from 1 to 5 identified disomic chromosomes, in crosses to a set of haploid strains collectively bearing 11 unmapped genes, the following chromosome assignments were obtained for these unmapped genes: arg80 on XIII;arg3 on X;car2 on XII; cpa1 and tsm8740 on XV; tsm7269 (=rna6) on II; cpa2 on X or XV; arg82 and tsm4572 on III, IV or XVI; car1 and arg81 on II, IV, VI, VII or XVI. Linkage tests between the unmapped genes and markers located on the chromosomes that had been designated as possible carriers by the previous analysis allowed 8 genes to be localized. The remaining three genes, cpa2, car1 and arg81 (located on fragment F8), could not be positioned on any of the chromosomes indicated by the trisomic analysis, in spite of testing for linkage to markers covering most of the known regions of these chromosomes.

摘要

利用一套携带 1 到 5 个已鉴定的二倍体染色体的酵母酿酒酵母的 8 个非整倍体菌株，与一套共同携带 11 个未定位基因的单倍体菌株杂交，得到了这些未定位基因在染色体上的以下分配：arg80 在 XIII；arg3 在 X；car2 在 XII；cpa1 和 tsm8740 在 XV；tsm7269（=rna6）在 II；cpa2 在 X 或 XV；arg82 和 tsm4572 在 III、IV 或 XVI；car1 和 arg81 在 II、IV、VI、VII 或 XVI。未定位基因与先前分析中被认为可能携带的染色体上的标记之间的连锁测试允许定位 8 个基因。其余三个基因 cpa2、car1 和 arg81（位于 F8 片段上），尽管测试了与覆盖这些染色体已知区域大部分的标记的连锁，但仍不能定位到三体分析所指示的任何染色体上。