Yamamoto K K, Gonzalez G A, Biggs W H, Montminy M R
Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, California 92037.
Nature. 1988 Aug 11;334(6182):494-8. doi: 10.1038/334494a0.
A nuclear protein, CREB, has been isolated from rat brain and shown to stimulate transcription of the cyclic AMP-responsive gene somatostatin as a dimer. Biochemical analysis suggests that dimerization and transcriptional efficacy of CREB protein in vitro are regulated by phosphorylation. These findings demonstrate that cellular signals can modulate gene expression by regulating the covalent modification of pre-existing nuclear factors.
一种核蛋白，即环磷腺苷反应元件结合蛋白（CREB），已从大鼠脑中分离出来，并被证明作为二聚体刺激环磷腺苷反应性基因生长抑素的转录。生化分析表明，CREB蛋白在体外的二聚化和转录效力受磷酸化调节。这些发现证明，细胞信号可以通过调节预先存在的核因子的共价修饰来调节基因表达。
