Song Jiao J, Tian Wen J, Kwok Lai-Yu, Wang Ya L, Shang Yi N, Menghe Bilige, Wang Jun G
Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Key Laboratory of Dairy Products Processing, Ministry of Agriculture, P. R. China, Department of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot, 010018,People's Republic of China.
Br J Nutr. 2017 Oct;118(7):481-492. doi: 10.1017/S0007114517002380.
The in vivo effects of administering free and microencapsulated Lactobacillus plantarum LIP-1 cells (2·0×109 colony-forming units/d) were evaluated in high-fat-diet-induced hyperlipidaemic rats. Results from real-time quantitative PCR targeting to LIP-1 cells showed a higher colon colonisation count of LIP-1 in the rats receiving microencapsulated cells compared with free cells (P<0·05). Moreover, the microencapsulated LIP-1 treatment resulted in a more obvious lipid-lowering effect (P<0·05). Meanwhile, their faecal samples had significantly less lipopolysaccharide-producing bacteria (especially Bilophila, Sutterella and Oscillibacter) and mucosa-damaging bacteria (Bilophila and Akkermansia muciniphila), whereas significantly more SCFA-producing bacteria (P<0·05) (namely Lactobacillus, Alloprevotella, Coprococcus, Eubacterium and Ruminococcus) and bacteria that potentially possessed bile salt hydrolase activity (Bacteroides, Clostridium, Eubacterium and Lactobacillus), and other beneficial bacteria (Alistipes and Turicibacter). Further, Spearman's correlation analysis showed significant correlations between some of the modulated gut bacteria and the serum lipid levels. These results together confirm that microcapsulation enhanced the colon colonisation of LIP-1 cells, which subsequently exhibited more pronounced effects in improving the gut microbiota composition of hyperlipidaemic rats and lipid reduction.
在高脂饮食诱导的高脂血症大鼠中评估了给予游离和微囊化植物乳杆菌LIP-1细胞（2.0×10⁹ 菌落形成单位/天）的体内效果。针对LIP-1细胞的实时定量PCR结果显示，与游离细胞相比，接受微囊化细胞的大鼠中LIP-1在结肠中的定殖数量更高（P<0.05）。此外，微囊化LIP-1处理导致更明显的降脂效果（P<0.05）。同时，它们的粪便样本中产生脂多糖的细菌（特别是嗜胆菌属、萨特氏菌属和颤杆菌属）和损害黏膜的细菌（嗜胆菌属和嗜黏蛋白阿克曼氏菌）显著减少，而产生短链脂肪酸的细菌（P<0.05）（即乳酸杆菌属、别普雷沃氏菌属、粪球菌属、真杆菌属和瘤胃球菌属）以及可能具有胆汁盐水解酶活性的细菌（拟杆菌属、梭菌属、真杆菌属和乳酸杆菌属）和其他有益细菌（阿里斯杆菌属和Turicibacter）显著增多。此外，Spearman相关性分析表明，一些被调节的肠道细菌与血清脂质水平之间存在显著相关性。这些结果共同证实，微囊化增强了LIP-1细胞在结肠中的定殖，随后在改善高脂血症大鼠的肠道微生物群组成和降低血脂方面表现出更显著的效果。
