Asif Sania, Malik Lubna
Department of Anatomy, University of Health Sciences, Lahore.
J Coll Physicians Surg Pak. 2017 Sep;27(9):536-539.
To see the effects of Nigella sativaon acetylsalicylic acid-induced nephrotoxicity in albino rats.
An experimental study.
The Anatomy Department of University of Health Sciences, Lahore, from January 2014 to December 2015.
Thirty-two female albino rats were divided into four groups. Group A(control) was given single dose of 10 mg/100 gm body weight of 1% methylcellulose, orally. Group B and C were treated with oral 1000 mg/kg acetylsalicylic acid as a single dose. Group D was given 250 mg/kg ethanolic extract of Nigella sativa(NSE) by oral gavage followed by single dose of 1000 mg/kg acetylsalicylic acid on 1st day of experiment; after that only NSE was continued till 7 days. Animals of groups A, C and D were sacrificed on day 8 and that of group B on day 2 of experiment. Cardiac puncture was performed to draw blood from each animal for renal function tests. Animals were evaluated for gross (Paired kidney weight, body weight, relative tissue body weight index) as well as for biochemical (Serum urea and creatinine) parameters.
Gross as well as biochemical parameters were markedly impaired in group B, but statistically significant improvement was noticed in Nigella sativatreated group. There was no self recovery in group C.
Ethanolic extract of Nigella sativahas a protective role against acetylsalicylic acid-induced nephrotoxicity in albino rats.
观察黑种草对乙酰水杨酸诱导的白化大鼠肾毒性的影响。
一项实验研究。
2014年1月至2015年12月，拉合尔健康科学大学解剖学系。
将32只雌性白化大鼠分为四组。A组（对照组）口服单剂量10mg/100g体重的1%甲基纤维素。B组和C组口服单剂量1000mg/kg乙酰水杨酸。D组在实验第1天经口灌胃给予250mg/kg黑种草乙醇提取物（NSE），随后给予单剂量1000mg/kg乙酰水杨酸；之后仅持续给予NSE直至第7天。A组、C组和D组的动物在第8天处死，B组的动物在实验第2天处死。进行心脏穿刺从每只动物采血进行肾功能测试。对动物进行大体（双肾重量、体重、相对组织体重指数）以及生化（血清尿素和肌酐）参数评估。
B组的大体及生化参数均明显受损，但在黑种草治疗组中观察到有统计学意义的改善。C组无自我恢复情况。
黑种草乙醇提取物对乙酰水杨酸诱导的白化大鼠肾毒性具有保护作用。
