Protective Effects of Nigella Sativaon Acetylsalicylic Acid-Induced Nephrotoxicity in Albino Rats.

OBJECTIVE

To see the effects of Nigella sativaon acetylsalicylic acid-induced nephrotoxicity in albino rats.

STUDY DESIGN

An experimental study.

PLACE AND DURATION OF STUDY

The Anatomy Department of University of Health Sciences, Lahore, from January 2014 to December 2015.

METHODOLOGY

Thirty-two female albino rats were divided into four groups. Group A(control) was given single dose of 10 mg/100 gm body weight of 1% methylcellulose, orally. Group B and C were treated with oral 1000 mg/kg acetylsalicylic acid as a single dose. Group D was given 250 mg/kg ethanolic extract of Nigella sativa(NSE) by oral gavage followed by single dose of 1000 mg/kg acetylsalicylic acid on 1st day of experiment; after that only NSE was continued till 7 days. Animals of groups A, C and D were sacrificed on day 8 and that of group B on day 2 of experiment. Cardiac puncture was performed to draw blood from each animal for renal function tests. Animals were evaluated for gross (Paired kidney weight, body weight, relative tissue body weight index) as well as for biochemical (Serum urea and creatinine) parameters.

RESULTS

Gross as well as biochemical parameters were markedly impaired in group B, but statistically significant improvement was noticed in Nigella sativatreated group. There was no self recovery in group C.

CONCLUSION

Ethanolic extract of Nigella sativahas a protective role against acetylsalicylic acid-induced nephrotoxicity in albino rats.