1Department of Anatomy, King Edward Medical University, Lahore, Pakistan.
2Department of Anatomy, Sahara Medical College, Narowal, Pakistan.
J Ayub Med Coll Abbottabad. 2021 Jan-Mar;33(1):39-43.
Aspirin is one of the popular, economical, easily accessible and commonly used drugs all over the world. Injudicious use of this over-the-counter available drug is a common cause of nephrotoxicity. The aim of the present study is to assess the protective effects of Nigella Sativa (NS) on the histology of kidneys against aspirin-induced toxicity. It was an experimental study that included comparative analysis of control and experimental groups, conducted in the department of Anatomy, University of Health Sciences, Lahore, from October 2016 to December 2016.
The study included thirty-two female albino rats which were equally distributed into 4 groups. Group A was run as control and given single oral dose of 1% methyl-cellulose (10mg/100gm body weight of rat). Group B and C were treated with a single oral dose of aspirin (1000mg/kg body weight) dissolved in 1% methyl-cellulose (10mg/100gm body weight). Group C animals were left untreated for 7 days. Group D was pre-treated on day 1 with oral dose of Nigella Sativa (NS) extract (250mg/kg body weight) followed one hour later by a single oral dose of aspirin (1000mg/kg body weight), subsequently NS extract was administered till day 7. Rats of group B were euthanized and dissected on 2nd day of experiment while those of groups A, C and D on 8th day. Kidneys were dissected out, weighed and fixed in 10% formalin. 5μm thick sections were yielded after tissue processing and stained with haematoxylin, eosin (H&E staining) and periodic acid Schiff's reagent (PAS staining). Histological parameters of distal convoluted tubules (DCT) were observed.
All histological parameters were normal in group A. Group B showed marked increase in epithelial necrosis, intraluminal protein casts and broken basement membranes of distal convoluted tubules. Group C showed no self-recovery. Statistically significant improvement was observed in the histology of distal convoluted tubules with treatment of Nigella Sativa extract in aspirin-ingested rats in group D.
Nigella Sativa extract has shown protective effects on kidneys against aspirin-induced damage as shown by improvement in the histological parameters of distal convoluted tubules.
阿司匹林是一种在全球范围内流行、经济实惠、易于获取且广泛使用的药物。不合理使用这种非处方药物是导致肾毒性的常见原因。本研究旨在评估黑种草（NS）对阿司匹林诱导的毒性引起的肾脏组织学的保护作用。这是一项实验研究，包括对照组和实验组的对比分析，于 2016 年 10 月至 12 月在拉合尔健康科学大学解剖系进行。
研究纳入 32 只雌性白化大鼠，平均分为 4 组。A 组作为对照组，给予单剂量的 1%甲基纤维素（10mg/100gm 大鼠体重）。B 组和 C 组给予单剂量的阿司匹林（1000mg/kg 体重）溶于 1%甲基纤维素（10mg/100gm 大鼠体重）。C 组动物在第 7 天未进行治疗。D 组于第 1 天给予黑种草（NS）提取物（250mg/kg 体重）口服剂量，1 小时后给予单剂量的阿司匹林（1000mg/kg 体重），随后直至第 7 天给予 NS 提取物。B 组大鼠在实验第 2 天处死并解剖，A、C 和 D 组大鼠在第 8 天处死并解剖。取出肾脏，称重，用 10%福尔马林固定。组织处理后产生 5μm 厚的切片，用苏木精、曙红（H&E 染色）和过碘酸希夫试剂（PAS 染色）染色。观察远端曲管（DCT）的组织学参数。
A 组所有组织学参数均正常。B 组显示出明显的上皮坏死、管腔内蛋白栓和远端曲管的基底膜破裂。C 组没有自行恢复。D 组阿司匹林摄入大鼠用黑种草提取物治疗后，DCT 的组织学得到了显著改善。
黑种草提取物对阿司匹林引起的肾脏损伤具有保护作用，表现在改善远端曲管的组织学参数上。
