Department of Materials Science and Engineering, Sharif University of Technology, P.O. Box, 11365-11155, Tehran, Iran.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, P.O. Box, 14155-6153, Tehran, Iran.
Sci Rep. 2017 Oct 10;7(1):12896. doi: 10.1038/s41598-017-13396-y.
Colloidal quantum dots (CQD) have attracted considerable attention for biomedical diagnosis and imaging as well as biochemical analysis and stem cell tracking. In this study, quasi core/shell lead sulfide/reduced graphene oxide CQD with near infrared emission (1100 nm) were prepared for potential bioimaging applications. The nanocrystals had an average diameter of ~4 nm, a hydrodynamic size of ~8 nm, and a high quantum efficiency of 28%. Toxicity assay of the hybrid CQD in the cultured human mononuclear blood cells does not show cytotoxicity up to 200 µg/ml. At high concentrations, damage to mitochondrial activity and mitochondrial membrane potential (MMP) due to the formation of uncontrollable amounts of intracellular oxygen radicals (ROS) was observed. Cell membrane and Lysosome damage or a transition in mitochondrial permeability were also noticed. Understanding of cell-nanoparticle interaction at the molecular level is useful for the development of new fluorophores for biomedical imaging.
胶体量子点 (CQD) 在生物医学诊断和成像以及生化分析和干细胞跟踪方面引起了相当大的关注。在这项研究中，制备了具有近红外发射 (1100nm) 的准核/壳型硫化铅/还原氧化石墨烯 CQD，用于潜在的生物成像应用。纳米晶体的平均直径约为 4nm，水动力直径约为 8nm，量子效率高达 28%。在培养的人单核血红细胞中，杂交 CQD 的毒性测定表明，其在高达 200μg/ml 的浓度下没有细胞毒性。在高浓度下，由于形成不可控数量的细胞内氧自由基 (ROS)，观察到线粒体活性和线粒体膜电位 (MMP) 的损伤。还注意到细胞膜和溶酶体损伤或线粒体通透性的转变。在分子水平上理解细胞-纳米颗粒相互作用对于开发用于生物医学成像的新型荧光团是有用的。
