Chung Bong Nam, Koh Sang Wook, Choi Kyung San, Joa Jae Ho, Kim Chun Hwan, Selvakumar Gopal
National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Korea.
Plant Pathol J. 2017 Oct;33(5):522-527. doi: 10.5423/PPJ.NT.01.2017.0019. Epub 2017 Oct 1.
We determined the effects of atmospheric temperature (10-30 ± 2°C in 5°C increments) and carbon dioxide (CO) levels (400 ± 50 ppm, 540 ± 50 ppm, and 940 ± 50 ppm) on the infection of cv. Chubaek by (PLRV). Below CO levels of 400 ± 50 ppm, the PLRV infection rate and RNA content in plant tissues increased as the temperature increased to 20 ± 2°C, but declined at higher temperatures. At high CO levels (940 ± 50 ppm), more plants were infected by PLRV at 30 ± 2°C than at 20 or 25 ± 2°C, whereas PLRV RNA content was unchanged in the 20-30 ± 2°C temperature range. The effects of atmospheric CO concentration on the acquisition of PLRV by and accumulation of PLRV RNA in plant tissues were investigated using a growth chamber at 20 ± 2°C. The PLRV RNA content slightly increased at elevated CO levels (940 ± 50 ppm), but this increase was not statistically significant. Transmission rates of PLRV by increased as CO concentration increased. More PLRV RNA accumulated in potato plants maintained at 540 or 940 ± 50 ppm CO, than in plants maintained at 400 ± 50 ppm. This is the first evidence of greater PLRV RNA accumulation and larger numbers of plants infected by PLRV under conditions of combined high CO levels (940 ± 50 ppm) and high temperature (30 ± 2°C).
我们测定了大气温度（10 - 30 ± 2°C，以5°C为增量）和二氧化碳（CO₂）水平（400 ± 50 ppm、540 ± 50 ppm和940 ± 50 ppm）对“秋白”品种感染马铃薯卷叶病毒（PLRV）的影响。在CO₂水平低于400 ± 50 ppm时，随着温度升高至20 ± 2°C，PLRV感染率和植物组织中的RNA含量增加，但在较高温度下下降。在高CO₂水平（940 ± 50 ppm）时，30 ± 2°C下感染PLRV的植株比20或25 ± 2°C时更多，而在20 - 30 ± 2°C温度范围内PLRV RNA含量不变。使用生长箱在20 ± 2°C下研究了大气CO₂浓度对蚜虫获取PLRV以及PLRV RNA在植物组织中积累的影响。在升高的CO₂水平（940 ± 50 ppm）下，蚜虫的PLRV RNA含量略有增加，但这种增加无统计学意义。随着CO₂浓度增加，蚜虫传播PLRV的比率升高。与保持在400 ± 50 ppm CO₂的植株相比，保持在540或940 ± 50 ppm CO₂的马铃薯植株中积累了更多的PLRV RNA。这是高CO₂水平（940 ± 50 ppm）和高温（30 ± 2°C）共同作用下PLRV RNA积累增加以及感染PLRV的蚜虫数量增多的首个证据。
