Dahal G, Hughes J d'A, Thottappilly G, Lockhart B E L
International Institute of Tropical Agriculture (IITA), Oyo Road, PMB 5320, Ibadan, Nigeria; International mailing address: IITA, c/o L. W. Lambourn & Co., Carolyn House, 26 Dingwall Road, Croydon CR9 3EE, UK.
Department of Plant Pathology, University of Minnesota, St. Paul 55108.
Plant Dis. 1998 Jan;82(1):16-21. doi: 10.1094/PDIS.1998.82.1.16.
The effect of temperature on symptom expression and detection of banana streak badnavirus (BSV) by immunosorbent electronmicroscopy (ISEM) and enzyme-linked immunosorbent assay of 12 in vitro-propagated plantain hybrids (genome AAB × AA), 3 ABB cooking banana, and 3 AAB plantain landraces was studied. Experiments were done for 2 years under two temperature regimes, 28 to 35°C in a screenhouse and 22°C in a temperature-controlled room. Most BSV-infected plants of plantain hybrids expressed symptoms under both conditions. Symptom expression was enhanced when plants were continuously grown at 22°C, but later became indiscernible when plants were continuously grown at 28 to 35°C. Plants grown at 22°C and showing severe symptoms contained significantly higher virus titer than plants grown at 28 to 35°C. When asymptomatic plants with very low virus titer at 28 to 35°C were transferred back to 22°C, there was a significant increase in both symptom severity and concentration of virus (greater than 3 to 5 times) in leaf tissues after 9 months. In contrast, the concentration of virus and symptom severity decreased in plants after transfer from 22°C to 28 to 35°C. Micropropagated plants of AAB plantain landrace cv. Mimi Abue and ABB cooking bananas (cvs. Bluggoe, Cardaba, and Pelipita) did not express visible symptoms under either temperature regime, but BSV was detected by ISEM in 23% of the plants. After 2 years at 22°C, virus was detected in 64% of the plants, but the concentration of virus remained low. Implications of these results on quarantine screening of in vitro plants and virus diagnosis are discussed.
研究了温度对 12 个离体繁殖的车前草杂交种（基因组 AAB×AA）、3 个 ABB 烹饪香蕉品种以及 3 个 AAB 车前草地方品种通过免疫吸附电子显微镜（ISEM）和酶联免疫吸附测定法来表达症状及检测香蕉条纹巴德诺病毒（BSV）的影响。实验在两种温度条件下进行了两年，一种是在防虫网室中 28 至 35°C，另一种是在温控室内 22°C。大多数感染 BSV 的车前草杂交种植株在两种条件下均表现出症状。当植株在 22°C 持续生长时症状表达增强，但当植株在 28 至 35°C 持续生长时症状后来变得难以辨认。在 22°C 生长且表现出严重症状的植株所含病毒滴度显著高于在 28 至 35°C 生长的植株。当在 28 至 35°C 病毒滴度极低的无症状植株转移回 22°C 时，9 个月后叶片组织中的症状严重程度和病毒浓度均显著增加（大于 3 至 5 倍）。相反，从 22°C 转移到 28 至 35°C 后，植株中的病毒浓度和症状严重程度降低。AAB 车前草地方品种 cv. Mimi Abue 和 ABB 烹饪香蕉品种（cv. Bluggoe、Cardaba 和 Pelipita）的微繁殖植株在两种温度条件下均未表现出可见症状，但通过 ISEM 在 23%的植株中检测到了 BSV。在 22°C 放置两年后，64%的植株检测到病毒，但病毒浓度仍然较低。讨论了这些结果对离体植物检疫筛选和病毒诊断的意义。
