Sun Hao-Yu, Li Quan, Liu Yu-Ying, Wei Xiao-Hong, Pan Chun-Shui, Fan Jing-Yu, Han Jing-Yan
Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking UniversityBeijing, China.
Tasly Microcirculation Research Center, Peking University Health Science CenterBeijing, China.
Front Physiol. 2017 Sep 22;8:729. doi: 10.3389/fphys.2017.00729. eCollection 2017.
Chronic stress induces endocrine disturbance, which contributes to the development of polycystic ovary syndrome (PCOS), a condition that remains a challenge for clinicians to cope with. The present study investigated the effect of Xiao-Yao-San (XYS), a traditional Chinese medicine formula used for treatment of gynecological disease, on the chronic stress-induced polycystic ovary and its underlying mechanism. Female Sprague-Dwaley rats underwent a 3 weeks chronic unpredictable mild stress (CUMS) procedure to establish the PCOS model, followed by 4 weeks treatment with XYS (0.505 g/kg or 1.01 g/kg) by gavage. Granulosa cells were exposed to noradrenaline (1 mM) for 24 h, followed by incubation with or without XYS-treated rat serum for 24 h. Post-treatment with XYS ameliorated CUMS-induced irregular estrous cycles and follicles development abnormalities, decrease of estradiol and progesterone level as well as increase of luteinizing hormone in serum, reduced cystic follicles formation and the apoptosis and autophagy of granulosa cells, attenuated the increase in dopamine beta hydroxylase and c-fos level in locus coeruleus, the noradrenaline level in serum and ovarian tissue, and the expression of beta 2 adrenergic receptor in ovarian tissue. Besides, XYS alleviated the reduction of phosphorylation of ribosomal protein S6 kinase polypeptide I and protein kinase B, as well as the increase of microtubule-associated protein light chain 3-I to microtubule-associated protein light chain 3-II conversion both and . This study demonstrated XYS as a potential strategy for CUMS induced polycystic ovary, and suggested that the beneficial role of XYS was correlated with the regulation of the sympathetic nerve activity.
慢性应激会引发内分泌紊乱，进而促使多囊卵巢综合征（PCOS）的发生，这一病症仍是临床医生面临的一大挑战。本研究探讨了用于治疗妇科疾病的中药方剂逍遥散（XYS）对慢性应激诱导的多囊卵巢的影响及其潜在机制。雌性Sprague-Dwaley大鼠接受为期3周的慢性不可预测轻度应激（CUMS）程序以建立PCOS模型，随后通过灌胃给予XYS（0.505 g/kg或1.01 g/kg）治疗4周。颗粒细胞暴露于去甲肾上腺素（1 mM）24小时，然后与经XYS处理或未处理的大鼠血清一起孵育24小时。XYS治疗后改善了CUMS诱导的不规则发情周期和卵泡发育异常、血清中雌二醇和孕酮水平降低以及促黄体生成素升高、减少了囊性卵泡形成以及颗粒细胞的凋亡和自噬，减弱了蓝斑中多巴胺β羟化酶和c-fos水平、血清和卵巢组织中去甲肾上腺素水平以及卵巢组织中β2肾上腺素能受体表达的增加。此外，XYS减轻了核糖体蛋白S6激酶多肽I和蛋白激酶B磷酸化的降低，以及微管相关蛋白轻链3-I向微管相关蛋白轻链3-II转化的增加。本研究证明XYS是治疗CUMS诱导的多囊卵巢的潜在策略，并表明XYS的有益作用与交感神经活动的调节有关。
