Matson Jyl S, Livny Jonathan, DiRita Victor J
Department of Medical Microbiology and Immunology, University of Toledo Medical School, Toledo, Ohio, United States of America.
Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, United States of America.
PLoS One. 2017 Oct 11;12(10):e0186199. doi: 10.1371/journal.pone.0186199. eCollection 2017.
The epidemic pathogen Vibrio cholerae senses and responds to different external stresses it encounters in the aquatic environment and in the human host. One stress that V. cholerae encounters in the host is exposure to antimicrobial peptides on mucosal surfaces. We used massively parallel cDNA sequencing (RNA-Seq) to quantitatively identify the transcriptome of V. cholerae grown in the presence and absence of sub-lethal concentrations of the antimicrobial peptide polymyxin B. We evaluated the transcriptome of both wild type V. cholerae and a mutant carrying a deletion of vc1639, a putative sensor kinase of an uncharacterized two-component system, under these conditions. In addition to many previously uncharacterized pathways responding with elevated transcript levels to polymyxin B exposure, we confirmed the predicted elevated transcript levels of a previously described LPS modification system in response to polymyxin B exposure. Additionally, we identified the V. cholerae homologue of visP (ygiW) as a regulatory target of VC1639. VisP is a conserved periplasmic protein implicated in lipid A modification in Salmonellae. This study provides the first systematic analysis of the transcriptional response of Vibrio cholerae to polymyxin B, raising important questions for further study regarding mechanisms used by V. cholerae to sense and respond to envelope stress.
霍乱弧菌这种流行性病原菌能感知并应对其在水生环境和人类宿主中遇到的不同外部压力。霍乱弧菌在宿主体内遇到的一种压力是暴露于粘膜表面的抗菌肽。我们使用大规模平行cDNA测序（RNA测序）来定量鉴定在存在和不存在亚致死浓度抗菌肽多粘菌素B的情况下生长的霍乱弧菌的转录组。在这些条件下，我们评估了野生型霍乱弧菌和携带vc1639缺失的突变体（vc1639是一个未表征的双组分系统的假定传感激酶）的转录组。除了许多以前未表征的途径在转录水平上对多粘菌素B暴露有升高反应外，我们还证实了先前描述的脂多糖修饰系统在多粘菌素B暴露时预测的转录水平升高。此外，我们鉴定出visP（ygiW）的霍乱弧菌同源物是VC1639的调控靶点。VisP是一种保守的周质蛋白，与沙门氏菌的脂质A修饰有关。这项研究首次对霍乱弧菌对多粘菌素B的转录反应进行了系统分析，提出了关于霍乱弧菌感知和应对包膜应激所使用机制的进一步研究的重要问题。
