Pinho Antonio Ivanildo, Oliveira Cláudia Sirlene, Lovato Fabricio Luís, Waczuk Emily Pansera, Piccoli Bruna Candia, Boligon Aline Augusti, Leite Nadghia Figueredo, Coutinho Henrique Douglas Melo, Posser Thais, Da Rocha João Batista Teixeira, Franco Jeferson Luis
a Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica . Universidade Federal de Santa Maria , Santa Maria , RS , Brazil.
b Departamento de Farmácia Industrial , Universidade Federal de Santa Maria , Santa Maria , RS , Brazil.
J Toxicol Environ Health A. 2017;80(23-24):1301-1313. doi: 10.1080/15287394.2017.1382408. Epub 2017 Oct 11.
Mercury (Hg) is widely distributed in the environment and is known to produce several adverse effects in organisms. The aim of the present study was to examine the in vitro antioxidant activity and Hg chelating ability of the hydroalcoholic extract of Psidium guajava leaves (HEPG). In addition, the potential protective effects of HEPG against Hg(II) were evaluated using a yeast model (Saccharomyces cerevisiae). HEPG was found to exert significant antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl scavenger and inhibition of lipid peroxidation induced by Fe(II) assays in a concentration-dependent manner. The extract also exhibited significant Hg(II) chelating activity. In yeast, Hg(II) induced a significant decrease in cell viability. In contrast, HEPG partially prevented the fall in cell viability induced by Hg(II). In conclusion, HEPG exhibited protective effects against Hg(II)-mediated toxicity, which may be related to both antioxidant and Hg(II)-chelating activities.
汞（Hg）广泛分布于环境中，已知会对生物体产生多种不利影响。本研究的目的是检测番石榴叶水醇提取物（HEPG）的体外抗氧化活性和汞螯合能力。此外，使用酵母模型（酿酒酵母）评估了HEPG对Hg(II)的潜在保护作用。在2,2-二苯基-1-苦基肼清除剂和铁（II）诱导的脂质过氧化抑制试验中，发现HEPG以浓度依赖性方式发挥显著的抗氧化活性。该提取物还表现出显著的Hg(II)螯合活性。在酵母中，Hg(II)导致细胞活力显著下降。相比之下，HEPG部分阻止了Hg(II)诱导的细胞活力下降。总之，HEPG对Hg(II)介导的毒性具有保护作用，这可能与抗氧化和Hg(II)螯合活性有关。
