Kakoyiannis C K, Winter P J, Marshall R B
Appl Environ Microbiol. 1984 Sep;48(3):545-9. doi: 10.1128/aem.48.3.545-549.1984.
Ninety-nine Campylobacter coli isolates were examined by bacterial restriction endonuclease DNA analysis (BRENDA) with HindIII. Isolates from poultry from the same environment had identical patterns, patterns of isolates carried by suckling piglets were generally the same as those of isolates recovered from their dams, and one human patient yielded the same BRENDA type when sampled 6 weeks later. The 14 human isolates examined produced 11 distinct BRENDA types. Forty-three C. coli isolates from pigs were represented by 20 BRENDA types. Ten C. coli isolates from the feces of gulls yielded five different BRENDA types. Thirty-two C. coli isolates from live chickens and processed chicken yielded five different BRENDA types. Three human isolates had identical DNA patterns; two were from brothers living in the same house, and the third was from a human with no apparent relationship to the brothers. Another human isolate was identical to a poultry isolate. None of the pig strains had DNA patterns resembling those of human strains, nor were the DNA patterns like those of any strains recovered from poultry or gulls. Four C. coli isolates were subcultured onto agar 23 times over a period of 45 days, and their BRENDA patterns were preserved. BRENDA shows great promise for use in epidemiological studies of C. coli.
采用HindIII通过细菌限制性内切酶DNA分析（BRENDA）对99株空肠弯曲菌进行了检测。来自相同环境家禽的分离株具有相同的图谱，哺乳仔猪携带的分离株图谱通常与其母畜分离株的图谱相同，一名人类患者在6周后采样时产生了相同的BRENDA类型。所检测的14株人类分离株产生了11种不同的BRENDA类型。来自猪的43株空肠弯曲菌分离株由20种BRENDA类型代表。来自鸥粪便的10株空肠弯曲菌分离株产生了5种不同的BRENDA类型。来自活鸡和加工鸡肉的32株空肠弯曲菌分离株产生了5种不同的BRENDA类型。三株人类分离株具有相同的DNA图谱；其中两株来自住在同一所房子里的兄弟，第三株来自与这两兄弟无明显关系的人。另一株人类分离株与一株家禽分离株相同。没有猪菌株的DNA图谱与人类菌株的相似，也不像从家禽或鸥中分离出的任何菌株的图谱。4株空肠弯曲菌分离株在45天内转接至琼脂上23次，其BRENDA图谱得以保留。BRENDA在空肠弯曲菌的流行病学研究中显示出巨大的应用前景。
