Ntasiou Panagiota, Kaldeli Kerou Alexandra, Karamanidou Theodora, Vlachou Afrodite, Tziros George T, Tsouknidas Alexander, Karaoglanidis George S
Laboratory of Plant Pathology, Faculty of Agriculture, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, P.O. Box 269, 54124 Thessaloniki, Greece.
PLiN Nanotechnology S.A., Spectra Business Center 12th km Thessaloniki-Chalkidiki, Thermi, 57001 Thessaloniki, Greece.
Nanomaterials (Basel). 2021 Jun 24;11(7):1667. doi: 10.3390/nano11071667.
Olive crop is frequently treated with copper fungicides to combat foliar and fruit diseases such as olive leaf spot caused by and anthracnose caused by spp. The replacement of copper-based products with more eco-friendly alternatives is a priority. Metal nanoparticles synthesized in several ways have recently revolutionized crop protection with applications against important crop pathogens. In this study, we present the development of four copper-based nanoparticles (CuNP Type 1 to 4) synthesized with a wet chemistry approach. The CuNPs were characterized using Transmission Electron Microscopy, Dynamic Light Scattering, Laser Doppler Electrophoresis, and Attenuated Total Reflection measurements. In addition, the activity of the four CuNP types was tested in vitro and in planta against and spp. In vitro sensitivity measurements showed that for both pathogens, mycelial growth was the most susceptible developmental stage to the tested compounds. Against both pathogens, CuNP Type 1 and Type 2 were found to be more active in reducing mycelial growth compared to the reference commercial compounds of copper oxide and copper hydroxide. In planta experiments showed that CuNP Type 3 and CuNP Type 4 exhibited a strong protectant activity against both and with control efficacy values significantly higher than those achieved by the applications of either reference product.
橄榄作物经常使用铜基杀菌剂来防治叶部和果实病害，如由[病原体名称1]引起的橄榄叶斑病和由[病原体名称2] spp.引起的炭疽病。用更环保的替代品取代铜基产品是当务之急。通过多种方式合成的金属纳米颗粒最近在作物保护方面引发了革命，可用于对抗重要的作物病原体。在本研究中，我们展示了通过湿化学方法合成的四种铜基纳米颗粒（1型至4型铜纳米颗粒）的研发过程。使用透射电子显微镜、动态光散射、激光多普勒电泳和衰减全反射测量对铜纳米颗粒进行了表征。此外，还在体外和植物体内测试了这四种类型铜纳米颗粒对[病原体名称1]和[病原体名称2] spp.的活性。体外敏感性测量表明，对于这两种病原体，菌丝体生长是对测试化合物最敏感的发育阶段。对于这两种病原体，与氧化铜和氢氧化铜的参考商业化合物相比，发现1型和2型铜纳米颗粒在抑制菌丝体生长方面更具活性。植物体内实验表明，3型和4型铜纳米颗粒对[病原体名称1]和[病原体名称2]均表现出强大的保护活性，其防治效果值显著高于使用任何一种参考产品所达到的效果。
