Department of Clinical Chemistry and Hematology, Zuyderland Medical Center, Heerlen, The Netherlands.
Laboratory for Clinical Thrombosis and Hemostasis, Department of Internal Medicine and Biochemistry, Cardiovascular Research Institute of Maastricht, Maastricht, The Netherlands.
Cytometry A. 2017 Nov;91(11):1059-1067. doi: 10.1002/cyto.a.23263. Epub 2017 Oct 11.
This study was performed to gain further insight in the heterogeneity of monocytes in the different categories of acute coronary syndrome (ACS), especially between patients with unstable angina pectoris, ST-elevation myocardial infarction (STEMI), and non-ST-elevation myocardial infarction (NSTEMI). For this purpose, blood samples were collected in the acute phase from patients presenting with an ACS. These samples were examined with multiparameter flow cytometry to identify the different monocyte subsets and to analyze the expression of monocyte-associated molecules. Leukocytes, as well as an absolute number of monocytes, showed a clear and significant increase in patients with STEMI. This increase was seen in all subtypes of monocytes. The classical monocytes (CD14++CD16-) of patients with an NSTEMI had a significantly increased CD11b expression when compared to the control group, while these cells showed a decreased expression pattern in STEMI patients. This increased CD11b-expression was also seen in the intermediate monocytes of NSTEMI, while it was almost completely downregulated on the intermediate monocytes of STEMI. Finally, CX3CR1, which is almost exclusively expressed on intermediate and nonclassical monocytes, showed a significant decrease in expression in patients with STEMI. In conclusion, intermediate and nonclassical monocytes have a different immunophenotypic pattern in patients with STEMI versus NSTEMI. These differences reflect the pro-inflammatory state of the monocytes in NSTEMI and can be used as target molecules for novel therapeutic strategies to diminish the migration of proinflammatory monocytes into the myocardial tissue. © 2017 International Society for Advancement of Cytometry.
本研究旨在深入了解不同类型急性冠状动脉综合征（ACS）中单核细胞的异质性，特别是不稳定型心绞痛、ST 段抬高型心肌梗死（STEMI）和非 ST 段抬高型心肌梗死（NSTEMI）患者之间的差异。为此，在 ACS 发作的急性期采集患者的血液样本。这些样本通过多参数流式细胞术进行检查，以识别不同的单核细胞亚群并分析单核细胞相关分子的表达。白细胞和单核细胞的绝对数量在 STEMI 患者中明显增加。这种增加见于所有单核细胞亚型。与对照组相比，NSTEMI 患者的经典单核细胞（CD14++CD16-）CD11b 表达明显增加，而 STEMI 患者的这些细胞表达模式下降。NSTEMI 的中间单核细胞也存在这种增加的 CD11b 表达，而 STEMI 的中间单核细胞几乎完全下调。最后，几乎仅在中间和非经典单核细胞上表达的 CX3CR1 在 STEMI 患者中的表达显著下降。总之，与 STEMI 患者相比，NSTEMI 患者的中间和非经典单核细胞具有不同的免疫表型模式。这些差异反映了 NSTEMI 中单核细胞的促炎状态，可作为新的治疗策略的靶标分子，以减少促炎单核细胞向心肌组织的迁移。 版权所有 2017 年国际细胞分析学会。
