Lem J, Chin A C, Thayer M J, Leach R J, Fournier R E
Department of Microbiology, University of Southern California School of Medicine, Los Angeles 90033.
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7302-6. doi: 10.1073/pnas.85.19.7302.
Tissue-specific extinguisher-1 (Tse-1) is a mouse genetic locus that can repress liver-specific tyrosine aminotransferase gene expression in trans. To search for other Tse-1-responsive genes, hepatoma microcell hybrids retaining mouse chromosome 11 or human chromosome 17, containing murine Tse-1 and human TSE1, respectively, were screened for expression of liver-specific mRNAs. While most liver gene activity was unaffected in such hybrids, phosphoenolpyruvate carboxykinase and tyrosine aminotransferase gene expression was coordinately repressed in these clones. Extinction of both genes was apparently mediated by a single genetic locus that resides on human chromosome 17.
组织特异性消除因子-1（Tse-1）是一个小鼠基因位点，它能够反式抑制肝脏特异性酪氨酸转氨酶基因的表达。为了寻找其他对Tse-1有反应的基因，对保留小鼠11号染色体或人类17号染色体（分别包含鼠源Tse-1和人源TSE1）的肝癌微细胞杂种进行了肝脏特异性mRNA表达筛选。虽然大多数肝脏基因活性在这类杂种中未受影响，但磷酸烯醇式丙酮酸羧激酶和酪氨酸转氨酶基因的表达在这些克隆中被协同抑制。这两个基因的消除显然是由位于人类17号染色体上的单个基因位点介导的。
