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一种用于检测流感血凝素抗原的单向放射免疫扩散技术。关于流感疫苗血凝素含量检测方法的建议。

A single-radial-immunodiffusion technique for the assay of influenza haemagglutinin antigen. Proposals for an assay method for the haemagglutinin content of influenza vaccines.

作者信息

Schild G C, Wood J M, Newman R W

出版信息

Bull World Health Organ. 1975;52(2):223-31.

PMID:816480
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2366365/
Abstract

Single-radial-diffusion techniques are proposed as possible alternatives to tests based on agglutination of erythrocytes for the assay of the haemagglutinin content of influenza vaccines. Two test procedures (microtest and macrotest) and the use of reference reagents to assay vaccines using these tests are described. The two tests are of similar reproducibility and accuracy but the macrotest is technically simpler to perform and results of assays may be obtained more rapidly.

摘要

单径向扩散技术被提议作为基于红细胞凝集的检测方法的可能替代方法，用于检测流感疫苗的血凝素含量。本文描述了两种检测程序（微量检测和常量检测）以及使用参考试剂通过这些检测来测定疫苗的方法。这两种检测方法具有相似的重现性和准确性，但常量检测在技术上操作更简单，并且可以更快地获得检测结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/730e/2366365/01952908eb15/bullwho00464-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/730e/2366365/a83924385e34/bullwho00464-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/730e/2366365/01952908eb15/bullwho00464-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/730e/2366365/a83924385e34/bullwho00464-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/730e/2366365/01952908eb15/bullwho00464-0100-a.jpg

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J Exp Med. 1954 Feb;99(2):183-99. doi: 10.1084/jem.99.2.183.
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Immunochemical quantitation of antigens by single radial immunodiffusion.通过单向辐射免疫扩散法对抗原进行免疫化学定量。
Immunochemistry. 1965 Sep;2(3):235-54. doi: 10.1016/0019-2791(65)90004-2.
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Crystalline antigen from the influenza virus envelope.来自流感病毒包膜的结晶抗原。
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