Department of Obstetrics and Gynaecology, University of Otago, Christchurch School of Medicine, Christchurch, New Zealand.
Nanotechnology. 2017 Nov 24;28(47):475101. doi: 10.1088/1361-6528/aa935e.
Nanomedicine has advanced the biomedical field with the availability of multifunctional nanoparticles (NPs) systems that can target a disease site enabling drug delivery and helping to monitor the disease. In this paper, we synthesised the gold nanoparticles (AuNPs) with an average size 18, 40, 60 and 80 nm, and studied the effect of nanoparticles size, concentration and incubation time on ovarian cancer cells namely, OVCAR5, OVCAR8, and SKOV3. The size measured by transmission electron microscopy images was slightly smaller than the hydrodynamic diameter; measured size by ImageJ as 14.55, 38.13, 56.88 and 78.56 nm. The cellular uptake was significantly controlled by the AuNPs size, concentration, and the cell type. The nanoparticles uptake increased with increasing concentration, and 18 and 80 nm AuNPs showed higher uptake ranging from 1.3 to 5.4 μg depending upon the concentration and cell type. The AuNPs were associated with a temporary reduction in metabolic activity, but metabolic activity remained more than 60% for all sample types; NPs significantly affected the cell proliferation activity in first 12 h. The increase in nanoparticle size and concentration induced the production of reactive oxygen species in 24 h.
纳米医学通过多功能纳米粒子（NPs）系统的可用性推动了生物医学领域的发展，这些系统可以靶向疾病部位，实现药物输送，并有助于监测疾病。在本文中，我们合成了平均尺寸为 18、40、60 和 80nm 的金纳米粒子（AuNPs），并研究了纳米粒子尺寸、浓度和孵育时间对卵巢癌细胞（即 OVCAR5、OVCAR8 和 SKOV3）的影响。通过透射电子显微镜图像测量的尺寸略小于水动力直径；通过 ImageJ 测量的尺寸分别为 14.55、38.13、56.88 和 78.56nm。纳米粒子的摄取量受纳米粒子的尺寸、浓度和细胞类型的显著控制。纳米粒子的摄取量随浓度的增加而增加，18nm 和 80nm 的 AuNPs 摄取量较高，范围为 1.3 至 5.4μg，取决于浓度和细胞类型。AuNPs 与代谢活性的暂时降低有关，但所有样本类型的代谢活性仍保持在 60%以上；纳米粒子在前 12 小时显著影响细胞增殖活性。在 24 小时内，纳米粒子尺寸和浓度的增加诱导了活性氧的产生。
