CircPro：一个用于识别具有蛋白质编码潜力的 circRNA 的综合工具。

CircPro: an integrated tool for the identification of circRNAs with protein-coding potential.

机构信息

Department of Bioinformatics; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.

出版信息

Bioinformatics. 2017 Oct 15;33(20):3314-3316. doi: 10.1093/bioinformatics/btx446.

DOI:10.1093/bioinformatics/btx446

PMID:29028266

Abstract

SUMMARY

Circular RNAs (circRNAs), a novel class of endogenous RNAs, are widespread in eukaryotic cells. Emerging roles in diverse biological processes suggest that circRNA is a promising key player in RNA world. Most circRNAs are generated through back-splicing of pre-mRNAs, forming a covalently closed loop structure with no 5' caps or 3' polyadenylated tails. In addition, most circRNAs were not associated with translating ribosomes, therefore, circRNAs were deemed to be noncoding. However, the latest research findings revealed that some circRNAs could generate proteins in vivo, which expands the landscape of transcriptome and proteome. To gain insights into the new area of circRNA translation, we introduce an integrated tool capable of detecting circRNAs with protein-coding potential from high-throughput sequencing data.

AVAILABILITY AND IMPLEMENTATION

CircPro is available at http://bis.zju.edu.cn/CircPro.

CONTACT

mchen@zju.edu.cn.

SUPPLEMENTARY INFORMATION

Supplementary data are available at Bioinformatics online.

摘要

环状 RNA（circRNA）是一类新型的内源性 RNA，广泛存在于真核细胞中。circRNA 在多种生物学过程中发挥着重要作用，这表明它可能是 RNA 世界中的一个关键调控因子。大多数 circRNA 是通过前体 mRNA 的反向剪接产生的，形成具有共价封闭环结构的分子，没有 5' 帽或 3' 多聚腺苷酸化尾巴。此外，大多数 circRNA 不与翻译核糖体结合，因此被认为是非编码 RNA。然而，最近的研究发现，一些 circRNA 可以在体内产生蛋白质，这扩展了转录组和蛋白质组的范围。为了深入了解 circRNA 翻译的新领域，我们介绍了一种集成工具，能够从高通量测序数据中检测具有蛋白编码潜力的 circRNA。