Sciarrone Danilo, Giuffrida Daniele, Rotondo Archimede, Micalizzi Giuseppe, Zoccali Mariosimone, Pantò Sebastiano, Donato Paola, Rodrigues-das-Dores Rosana Goncalves, Mondello Luigi
Dipartimento di "Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali", University of Messina, Polo Annunziata, Viale Annunziata, 98168 Messina, Italy.
Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali, University of Messina, Polo Annunziata, c/o PanLab, viale Annunziata, 98168 Messina, Italy.
J Chromatogr A. 2017 Nov 17;1524:246-253. doi: 10.1016/j.chroma.2017.10.007. Epub 2017 Oct 6.
Cordia verbenacea D.C. (Boraginaceae, Varronia curassavica Jacq. synonym) is a medicinal plant, native from Brazil, especially the leaves are used in folk medicine. The aim of this study was to extend the characterization of the volatile fraction of the essential oil obtained from this plant, by using GC-FID, GC-MS, and chiral GC. Moreover, to further clarify the composition of the volatile fraction, preparative multidimensional-GC (prep-MDGC) was used to collect unknown compounds, followed by NMR characterization. Specifically, the chemical characterization, both qualitative and quantitative, of the volatile fraction of the essential oil obtained from Cordia verbenacea cultivated in the Minas Gerais area (central area of Brazil) was investigated for the first time. The principal components from a quantitative point of view were α-pinene (25.32%; 24.48g/100g) and α-santalene (17.90%; 17.30g/100g), belonging to the terpenes family. Chiral-GC data are reported for the enantiomeric distribution of 7 different components. Last, to obtain the complete characterization of the essential oil constituents, prep-MDGC analysis was used to attain the isolation of two compounds, not present in the principal MS databases, which were unambiguously identified by NMR investigation as (E)-α-santalal and (E)-α-bergamotenal, reported for the first time in Cordia verbenacea essential oil.
马鞭草科破布木属的巴西破布木（Cordia verbenacea D.C.，异名：Varronia curassavica Jacq.）是一种药用植物，原产于巴西，尤其其叶子在民间医学中被使用。本研究的目的是通过气相色谱 - 氢火焰离子化检测器（GC - FID）、气相色谱 - 质谱联用仪（GC - MS）和手性气相色谱法，扩展对该植物所获精油挥发性成分的表征。此外，为进一步阐明挥发性成分的组成，采用制备型多维气相色谱（prep - MDGC）收集未知化合物，随后进行核磁共振（NMR）表征。具体而言，首次对巴西米纳斯吉拉斯地区（巴西中部地区）种植的巴西破布木所获精油挥发性成分进行了定性和定量的化学表征。从定量角度来看，主要成分是属于萜类家族的α - 蒎烯（25.32%；24.48克/100克）和α - 檀香烯（17.90%；17.30克/100克）。报告了手性气相色谱关于7种不同成分对映体分布的数据。最后，为获得精油成分的完整表征，使用制备型多维气相色谱分析分离出两种在主要质谱数据库中不存在的化合物，通过核磁共振研究明确鉴定为（E）-α - 檀香醛和（E）-α - 香柠檬烯醛，这两种化合物首次在巴西破布木精油中被报道。
