Zhang Guanshi, Tobolski Dawid, Zwierzchowski Grzegorz, Mandal Rupasri, Wishart David S, Ametaj Burim N
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Center for Renal Precision Medicine, Division of Nephrology, Department of Medicine, The University of Texas Health, San Antonio, TX 78229, USA.
Metabolites. 2021 Sep 17;11(9):633. doi: 10.3390/metabo11090633.
The retained placenta is a common pathology of dairy cows. It is associated with a significant drop in the dry matter intake, milk yield, and increased susceptibility of dairy cows to metritis, mastitis, and displaced abomasum. The objective of this study was to identify metabolic alterations that precede and are associated with the disease occurrence. Blood samples were collected from 100 dairy cows at -8 and -4 weeks prior to parturition and on the day of retained placenta, and only 16 healthy cows and 6 cows affected by retained placenta were selected to measure serum polar metabolites by a targeted gas chromatography-mass spectroscopy (GC-MS) metabolomics approach. A total of 27 metabolites were identified and quantified in the serum. There were 10, 18, and 17 metabolites identified as being significantly altered during the three time periods studied. However, only nine metabolites were identified as being shared among the three time periods including five amino acids (Asp, Glu, Ser, Thr, and Tyr), one sugar (myo-inositol), phosphoric acid, and urea. The identified metabolites can be used as predictive biomarkers for the risk of retained placenta in dairy cows and might help explain the metabolic processes that occur prior to the incidence of the disease and throw light on the pathomechanisms of the disease.
胎衣不下是奶牛常见的病理状况。它与干物质采食量显著下降、产奶量降低以及奶牛患子宫炎、乳腺炎和真胃移位易感性增加有关。本研究的目的是确定在疾病发生之前并与之相关的代谢变化。在分娩前8周和4周以及胎衣不下当天从100头奶牛采集血样，仅选择16头健康奶牛和6头受胎衣不下影响的奶牛，采用靶向气相色谱 - 质谱（GC-MS）代谢组学方法测定血清极性代谢物。血清中共鉴定并定量了27种代谢物。在所研究的三个时间段内分别有10种、18种和17种代谢物被鉴定为有显著变化。然而，仅9种代谢物在三个时间段中均存在变化，包括5种氨基酸（天冬氨酸、谷氨酸、丝氨酸、苏氨酸和酪氨酸）、1种糖类（肌醇）、磷酸和尿素。所鉴定的代谢物可作为奶牛胎衣不下风险的预测生物标志物，并可能有助于解释疾病发生前的代谢过程，阐明该疾病的发病机制。
