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新种和新种,从厦门海岸分离得到。

sp. nov. and sp. nov., isolated from the Xiamen coast.

机构信息

School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.

Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of PR China; State Key Laboratory Breeding Base of Marine Genetic Resources; Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.

出版信息

Int J Syst Evol Microbiol. 2019 Oct;69(10):3100-3108. doi: 10.1099/ijsem.0.003595.

Abstract

Strain GCL-8 and GCL-11 were isolated from Guanyinshan, a sandy beach on the Xiamen coast. Cells of the two strains were Gram-stain-negative, catalase-positive, oxidase-positive, rod-shaped and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains GCL-8 and GCL-11 belonged to the genera and , with the highest sequence similarities to B108 (96.5%) and CGMCC 1.12174 (97.3%), respectively. The major fatty acids of strain GCL-8 were summed feature 8 (Cω7/ω6), Ccyclo ω8, C and C, while the major fatty acids of strain GCL-11 were iso-C G, iso-C and iso-C 3-OH. The G+C contents of the chromosomal DNA of strains GCL-8and GCL-11 were 64.1 mol% and 45.67 mol%, respectively. The respiratory quinones of strains GCL-8 and GCL-11 were Q-10 and MK-6, respectively. The polar lipids of strain GCL-8 were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, one unidentified aminolipid, three unidentified phospholipids and three unidentified polar lipids. The polar lipids of strain GCL-11were phosphatidylethanolamine, seven unidentified phospholipids and four unidentified polar lipids. The combined genotypic and phenotypic data show that strains GCL-8 and GCL-11 represent two novel species within genera and , for which the names sp. nov. and sp. nov. are proposed, with type strains GCL-8 (=MCCC 1A11651 = KCTC 52430) and GCL-11 (=MCCC 1A11649=KCTC 52432).

摘要

菌株 GCL-8 和 GCL-11 从厦门海岸观音山的沙滩中分离得到。这两株菌的细胞革兰氏染色阴性,接触酶阳性,氧化酶阳性,呈杆状且不运动。基于 16S rRNA 基因序列的系统发育分析表明,菌株 GCL-8 和 GCL-11 属于 和 属,与 B108(96.5%)和 CGMCC 1.12174(97.3%)的序列相似性最高。菌株 GCL-8 的主要脂肪酸为 sum feature 8(Cω7/ω6)、Ccyclo ω8、C 和 C,而菌株 GCL-11 的主要脂肪酸为 iso-C G、iso-C 和 iso-C 3-OH。菌株 GCL-8 和 GCL-11 的染色体 DNA G+C 含量分别为 64.1 mol%和 45.67 mol%。菌株 GCL-8 和 GCL-11 的呼吸醌分别为 Q-10 和 MK-6。菌株 GCL-8 的极性脂为二磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的胺磷脂、一种未鉴定的胺脂、三种未鉴定的磷脂和三种未鉴定的极性脂。菌株 GCL-11 的极性脂为磷脂酰乙醇胺、七种未鉴定的磷脂和四种未鉴定的极性脂。综合表型和基因型数据表明,菌株 GCL-8 和 GCL-11 代表 和 属中的两个新种,分别命名为 sp. nov. 和 sp. nov.,其模式菌株分别为 GCL-8(=MCCC 1A11651 = KCTC 52430)和 GCL-11(=MCCC 1A11649 = KCTC 52432)。

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