School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of PR China; State Key Laboratory Breeding Base of Marine Genetic Resources; Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3100-3108. doi: 10.1099/ijsem.0.003595.
Strain GCL-8 and GCL-11 were isolated from Guanyinshan, a sandy beach on the Xiamen coast. Cells of the two strains were Gram-stain-negative, catalase-positive, oxidase-positive, rod-shaped and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains GCL-8 and GCL-11 belonged to the genera and , with the highest sequence similarities to B108 (96.5%) and CGMCC 1.12174 (97.3%), respectively. The major fatty acids of strain GCL-8 were summed feature 8 (Cω7/ω6), Ccyclo ω8, C and C, while the major fatty acids of strain GCL-11 were iso-C G, iso-C and iso-C 3-OH. The G+C contents of the chromosomal DNA of strains GCL-8and GCL-11 were 64.1 mol% and 45.67 mol%, respectively. The respiratory quinones of strains GCL-8 and GCL-11 were Q-10 and MK-6, respectively. The polar lipids of strain GCL-8 were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, one unidentified aminolipid, three unidentified phospholipids and three unidentified polar lipids. The polar lipids of strain GCL-11were phosphatidylethanolamine, seven unidentified phospholipids and four unidentified polar lipids. The combined genotypic and phenotypic data show that strains GCL-8 and GCL-11 represent two novel species within genera and , for which the names sp. nov. and sp. nov. are proposed, with type strains GCL-8 (=MCCC 1A11651 = KCTC 52430) and GCL-11 (=MCCC 1A11649=KCTC 52432).
菌株 GCL-8 和 GCL-11 从厦门海岸观音山的沙滩中分离得到。这两株菌的细胞革兰氏染色阴性，接触酶阳性，氧化酶阳性，呈杆状且不运动。基于 16S rRNA 基因序列的系统发育分析表明，菌株 GCL-8 和 GCL-11 属于 和 属，与 B108（96.5%）和 CGMCC 1.12174（97.3%）的序列相似性最高。菌株 GCL-8 的主要脂肪酸为 sum feature 8（Cω7/ω6）、Ccyclo ω8、C 和 C，而菌株 GCL-11 的主要脂肪酸为 iso-C G、iso-C 和 iso-C 3-OH。菌株 GCL-8 和 GCL-11 的染色体 DNA G+C 含量分别为 64.1 mol%和 45.67 mol%。菌株 GCL-8 和 GCL-11 的呼吸醌分别为 Q-10 和 MK-6。菌株 GCL-8 的极性脂为二磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的胺磷脂、一种未鉴定的胺脂、三种未鉴定的磷脂和三种未鉴定的极性脂。菌株 GCL-11 的极性脂为磷脂酰乙醇胺、七种未鉴定的磷脂和四种未鉴定的极性脂。综合表型和基因型数据表明，菌株 GCL-8 和 GCL-11 代表 和 属中的两个新种，分别命名为 sp. nov. 和 sp. nov.，其模式菌株分别为 GCL-8（=MCCC 1A11651 = KCTC 52430）和 GCL-11（=MCCC 1A11649 = KCTC 52432）。
