Wang Ying, Zhang Jing, Zhang Yue, Huang Danyi, Zhao Jian, Li Gang, Lei Ying
Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Stem Cell Res. 2017 Oct;24:33-35. doi: 10.1016/j.scr.2017.08.008. Epub 2017 Aug 12.
Peripheral blood mononuclear cells (PBMC) were collected from a 65-year old healthy woman with Chinese Han genetic background. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously toward the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. The iPSC line can be used as control in disease mechanism studies. Resource table.
从一名具有中国汉族遗传背景的65岁健康女性身上采集外周血单个核细胞（PBMC）。使用非整合型附加体质粒载体系统，利用人类OKSM转录因子对PBMC进行重编程。通过对多能性标志物进行免疫细胞化学检测，证实无转基因的诱导多能干细胞（iPSC）具有多能性，并且在体外可自发分化为三个胚层。此外，该iPSC系显示出正常的核型。该iPSC系可用于疾病机制研究中的对照。资源表。
